Memorias do Instituto Oswaldo Cruz · 2026
Entomo-virological investigation during the epizootic outbreak of sylvatic yellow fever in Rio Grande do Sul, Brazil, between 2021 and 2022.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Müller Nícolas Felipe Drumm, Lima Marcelo de Moura, Dos Santos Edmilson, Campos Aline Alves Scarpellini, Menegazzi Thomas Rosa, Melgarejo Alanis Silva, Paredes-Galarza Bruna, Violet-Lozano Lina, Oliveira Martha Trindade, Oliveira Cirilo Henrique, Roehe Paulo Michel, Campos Fabrício Souza, de Abreu Filipe Vieira Santos, Cardoso Jáder da Cruz, Franco Ana Cláudia
Аннотация
BACKGROUND: Yellow fever virus (YFV) re-emerged among non-human primates (NHPs) in Rio Grande do Sul in early 2021, more than a decade after its last detection in the state. The spread of the virus was accompanied by increased mortality among NHPs.
OBJECTIVES: To conduct entomological surveillance and molecular detection of YFV and other Orthoflavivirus species in mosquito samples collected from affected and potentially receptive areas.
METHODS: Mosquitoes were collected during epizootics using human landing catches, BG-Pro traps, and ovitraps. Virus detection was performed using reverse transcription real-time polymerase chain reaction (RT-qPCR) assays targeting YFV and pan-Orthoflavivirus sequences.
FINDINGS: A total of 1,210 mosquitoes, representing 26 taxa, were collected across 17 municipalities. Psorophora ferox was the most abundant species, followed by Culex (Culex) spp., accounting for 27% and 12% of the specimens, respectively. Haemagogus leucocelaenus, the primary YFV vector in the region, was also among the most frequently captured species, representing 7%. In total, 203 mosquito pools were assembled by species, location, and date of collection. RT-qPCR analysis did not detect YFV or other Orthoflavivirus RNA in any of the samples.
MAIN CONCLUSIONS: Although mosquitoes were collected during a period of active YFV circulation, the absence of virus detection suggests that arboviral circulation in vector populations may occur at low frequencies, even during outbreaks.
OBJECTIVES: To conduct entomological surveillance and molecular detection of YFV and other Orthoflavivirus species in mosquito samples collected from affected and potentially receptive areas.
METHODS: Mosquitoes were collected during epizootics using human landing catches, BG-Pro traps, and ovitraps. Virus detection was performed using reverse transcription real-time polymerase chain reaction (RT-qPCR) assays targeting YFV and pan-Orthoflavivirus sequences.
FINDINGS: A total of 1,210 mosquitoes, representing 26 taxa, were collected across 17 municipalities. Psorophora ferox was the most abundant species, followed by Culex (Culex) spp., accounting for 27% and 12% of the specimens, respectively. Haemagogus leucocelaenus, the primary YFV vector in the region, was also among the most frequently captured species, representing 7%. In total, 203 mosquito pools were assembled by species, location, and date of collection. RT-qPCR analysis did not detect YFV or other Orthoflavivirus RNA in any of the samples.
MAIN CONCLUSIONS: Although mosquitoes were collected during a period of active YFV circulation, the absence of virus detection suggests that arboviral circulation in vector populations may occur at low frequencies, even during outbreaks.