Utility of liquid-based cytology and cell block obtained by vitrectomy under infusion to diagnose vitreoretinal lymphoma.

PURPOSE: To confirm the definitive diagnostic method of vitreoretinal lymphoma by vitrectomy under infusion.

STUDY

DESIGN

Retrospective, pathological analysis

METHODS

Twenty-two patients participated in this study; 29 vitreous samples were obtained under infusion. Liquid-based cytology (LBC) was performed by targeting areas with a high cell concentration while observing the surgical field under infusion. Cell block analysis was performed by the vitreous cell components in the intraocular irrigating fluid.

RESULTS

Our vitreous sample collection did not result in adverse events. In LBC samples, atypical large lymphoid cells were identified in 89.7%. These samples contained between 30 and over 10,000 atypical large lymphoid cells. Necrosis was observed in 76.9% in the background of LBC samples. In cell block samples, atypical large lymphoid cells were identified in 87.0%. Immunohistochemical staining revealed the following immunophenotypes for the lymphoid cells: CD20 (100%), CD79a (100%), CD10 (0%), BCL6 (57.1%), MUM1 (85.7%), and Ki-67 LI 66.3±12.7%.

CONCLUSION

This method ensures safety and high diagnostic accuracy, thereby contributing to the definitive diagnosis of vitreoretinal lymphoma.