Current microbiology · 2025
A New Vaccine Candidate Expressing JUNV GP1-GP2 Against Argentine Hemorrhagic Fever Based on Baculovirus Surface Display.
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Tomatis Carla, Aguiar Mara C A M, Charo Nancy, Aquila Silvia, Thomas Pablo D, Arrías Paula N, Pidre Matías L, Romanowski Víctor, Riera Laura, Brignone Julia, Maiza Andrea, Carrera Silva Eugenio Antonio, Ferrer María Florencia, Gómez Ricardo Martín
Аннотация
With the aim of developing a new vaccine candidate using a recombinant baculovirus (rBV) expressing the Junín virus (JUNV) GP1 and GP2 proteins on its surface, a pBacPAK9 vector containing the BV-GP64 signal peptide, the JUNV GP1-GP2 and the transmembrane domain of the vesicular stomatitis virus G (VSV-G) protein were synthesized. This plasmid was co-transfected with the bApGOZA bacmid into Sf9 cells. The rBV was recovered from the supernatant. Protein expression was confirmed with a human serum by Western blot and with an anti-GPC monoclonal antibody by IFI staining and virometry. In an ELISA test, mice immunized with rBV exhibited specific antibody levels that also showed neutralizing properties in a plaque reduction test compared to controls. Guinea pigs immunized with rBV and challenged with JUNV showed partial protection compared to the Candid#1 vaccine. We have developed an rBV surface display platform to validate a potential JUNV vaccine candidate.