Chapare Hemorrhagic Fever and Virus Detection in Rodents in Bolivia in 2019.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Loayza Mafayle Roxana, Morales-Betoulle Maria E, Romero Carla, Cossaboom Caitlin M, Whitmer Shannon, Alvarez Aguilera Carlos E, Avila Ardaya Cinthia, Cruz Zambrana Mirian, Dávalos Anajia Andrés, Mendoza Loayza Nelly, Montaño Ana-Maria, Morales Alvis Fernando L, Revollo Guzmán Jimmy, Sasías Martínez Sebastián, Alarcón De La Vega Gricel, Medina Ramírez Armando, Molina Gutiérrez Jhemis T, Cornejo Pinto Alex J, Salas Bacci Renata, Brignone Julia, Garcia Jorge, Añez Arletta, Mendez-Rico Jairo, Luz Kleber, Segales Ariel, Torrez Cruz Karen M, Valdivia-Cayoja Adolfo, Amman Brian R, Choi Mary J, Erickson Bobbie-Rae, Goldsmith Cynthia, Graziano James C, Joyce Allison, Klena John D, Leach Austin, Malenfant Jason H, Nichol Stuart T, Patel Ketan, Sealy Tara, Shoemaker Trevor, Spiropoulou Christina F, Todres Alison, Towner Jonathan S, Montgomery Joel M
Аннотация
METHODS: We obtained samples for next-generation sequencing and virus isolation. Human and rodent specimens were tested by means of virus-specific real-time quantitative reverse-transcriptase-polymerase-chain-reaction assays, next-generation sequencing, and virus isolation.
RESULTS: Nine cases of hemorrhagic fever were identified; four of the patients with this illness died. The etiologic agent was identified as Mammarenavirus Chapare mammarenavirus, or Chapare virus (CHAPV), which causes Chapare hemorrhagic fever (CHHF). Probable nosocomial transmission among health care workers was identified. Some patients with CHHF had neurologic manifestations, and those who survived had a prolonged recovery period. CHAPV RNA was detected in a variety of human body fluids (including blood; urine; nasopharyngeal, oropharyngeal, and bronchoalveolar-lavage fluid; conjunctiva; and semen) and in specimens obtained from captured small-eared pygmy rice rats (Oligoryzomys microtis). In survivors of CHHF, viral RNA was detected up to 170 days after symptom onset; CHAPV was isolated from a semen sample obtained 86 days after symptom onset.
CONCLUSIONS: M. Chapare mammarenavirus was identified as the etiologic agent of CHHF. Both spillover from a zoonotic reservoir and possible person-to-person transmission were identified. This virus was detected in a rodent species, O. microtis. (Funded by the Bolivian Ministry of Health and others.).