Characterization of human mesenchymal stem cell according to delivery route and its correlation with clinical-gynecological history.

OBJECTIVE

To assess the pluripotency profile of human mesenchymal stem cells (hMSCs) as influenced by delivery mode and obstetric-gynecological background.

METHODS

Thirty-nine placentas were included. A fragment of the AM (5 cm) was utilized to obtain the AM-hMSCs. The cell cultures were monitored until 90% confluence, before carrying out expansion (passages). The cultures, cell morphology, adhesion, immunophenotyping characterization, and differentiation capacity were evaluated and compared with hMSC from bone marrow (BM-hMSC). T-student and Mann-Whitney U tests were carried out. Statistical significance was set at a p < 0.05.

RESULTS

No differences were observed in the proliferation and expansion of AM-hMSC obtained by vaginal or cesarean delivery route. Similar results were observed in the immunological characterization; however, CD105 was significantly lower compared with BM-hMSC. Nevertheless, cells from vaginal or cesarean delivery route showed great differentiation capacity to adipogenic, chondrogenic, and osteogenic lineages.

CONCLUSIONS

The delivery route, clinical data, and obstetric and gynecologic histories are no limitations for using the AM to obtain hMSC and its possible application in Regenerative Medicine.