Wound healing efficacy of Couroupita Guianensis loaded carboxymethylcellulose film.

Couroupita Guianensis extracts loaded carboxymethylcellulose film (CGELCF) was fabricated through a solution casting technique. Phytochemical screening confirmed the presence of alkaloids, proteins, flavonoids, saponins, carbohydrates, cardiac glycosides, and coumarins in CGE. The HPLC revealed that ellagic acid and gallic acid derivatives are abundant in CGE. FTIR spectrum confirmed the different functional group's presence in the film. The XRD, DSC, and TGA revealed the crystallinity, thermal stability, melting point (300 °C), and degradation of the film. FESEM and EDX analysis revealed surface morphology and elemental compositions. The atomic wt% of C, O, Na, and P were found as follows 38.39, 49.34, 11.44, and 0.83. The zone of inhibition was found as 19 and 20 mm against Staphylococcus aureus and Escherichia coli respectively. The tensile strength and modulus of elasticity was found 7.35 MPa and 1.38 GPa respectively. In-vitro wound scratch revealed above 90 % of cell migrations for all the concentrations after 18 h. The in-vitro cytotoxicity revealed 90.83 % of cell viability and 9.17 % of toxicity. In-vivo studies revealed that wound closure and wound healing with the CGELCF mice was within 7 days and exhibited the rapid drug. The CGELCF may find usage as a bandage for wounds.