Immunoglobulin Light-Chain Amyloidosis: Clinical Presentations and Diagnostic Approach.

Systemic immunoglobulin light-chain (AL) amyloidosis is caused by plasma cell clones in the bone marrow (median 7%–10% marrow infiltration) that produce insoluble, misfolded immunoglobulin light chain proteins, which are deposited in various tissues and organs as amyloid fibrils leading to progressive organ dysfunction ( Gertz, 2016 ; Grogan, Dispenzieri, & Gertz, 2017 ; Kastritis & Dimopoulos, 2016 ). This systemic disease is a rare disorder, with an estimated annual incidence of 6 to 10 per million person-years in the United Kingdom and United States ( Banypersad, Moon, Whelan, Hawkins, & Wechalekar, 2012 ; Comenzo, 2007a , 2007b ; Merlini & Palladini, 2008 ). The actual number of patients with this disorder may be higher due to underdiagnosis, with a recent real-world epidemiological study estimating an incidence of up to 14 per million person-years in the United States ( Quock, Yan, Chang, Guthrie, & Broder, 2018 ). AL amyloidosis is a disease that occurs in adults and is predominantly seen in the sixth decade of life (median age at diagnosis being estimated as 60–63 years); however, amyloidosis has been diagnosed in patients as young as 40 and is more prevalent in male patients ( Abeykoon et al., 2017 ; Comenzo, 2007a , 2007b ; Merlini & Palladini, 2008 ). There are approximately 30 different types of amyloidogenic proteins that may cause systemic or localized disease ( Sipe et al., 2014 ), and AL amyloidosis is one of the most common forms of systemic disease ( Palladini & Merlini, 2016 ). Signs and symptoms of AL amyloidosis are dependent on the involved organs and severity of organ damage. Initial symptoms are nonspecific, vary widely, and often overlap with those arising from other common diseases ( Gertz, 2016 ; Grogan et al., 2017 ; Lousada, Comenzo, Landau, Guthrie, & Merlini, 2015 ; Palladini & Merlini, 2016 ). Consequently, the diagnosis of this rare condition represents a challenge for clinicians.

Data from a patient experience survey by the Amyloidosis Research Consortium, which included 533 patients with amyloidosis (72% AL), showed that 37% of patients did not receive their definitive diagnosis of amyloidosis until ≥ 1 year from the initial onset of symptoms, with 32% requiring visits to ≥ 5 physicians before establishing the diagnosis of amyloidosis, and 34% of patients were diagnosed by hematology/oncology specialists ( Lousada et al., 2015 ). Other reports have also noted substantial delays in the diagnosis of AL amyloidosis ( McCausland et al., 2018 ; Muchtar et al., 2016 ) associated with the challenges of nonspecific symptoms and misdiagnosis. In a longitudinal, noninterventional study of community-based patients with AL amyloidosis, patients reported an average of 3 years from symptom onset to diagnosis ( McCausland et al., 2018 ).

These delays in diagnosis have a significant impact on patients as treatment outcomes are poorer in patients who experience a delay in diagnosis compared with those who achieve early diagnosis ( Grogan et al., 2017 ). This review highlights the need for early recognition of clinical presentations and diagnostic approach for systemic AL amyloidosis specifically, summarizing the key presenting symptoms that clinicians need to be alert to, and discussion of the latest diagnostic tests, with the aim of expediting symptom identification and diagnosis.

THE IMPORTANCE OF EARLY DIAGNOSIS OF AL AMYLOIDOSIS

Establishing an early diagnosis of AL amyloidosis is important because it enables treatment to be started early in the disease course, with the aim of reducing the burden of the free light-chain (FLC) producing plasma cell clone, thereby preventing further organ damage ( Merlini & Palladini, 2012 ). A high percentage of bone marrow plasma cells and baseline FLC burden at diagnosis predict poor survival, and a reduction in FLC with therapy is associated with improved outcomes ( Dispenzieri et al., 2006 ; Kourelis et al., 2013 ; Kumar et al., 2010 ; Lachmann et al., 2003 ). The spectrum and severity of organ involvement also have a great impact on prognosis and survival ( Kyle, Greipp, & O’Fallon, 1986 ). Although autologous peripheral blood stem cell transplantation (ASCT) is an effective therapy for AL amyloidosis, with a 10-year survival rate of 43% ( Sidiqi et al., 2018 ), the majority of patients are ineligible for this aggressive treatment due to significant organ dysfunction or comorbidities.

Frequency of Common Organ Involvement

Multisystem organ involvement is the hallmark of AL amyloidosis. In a single-center series, Merlini and Palladini reported that 68% of patients had more than one organ involved at diagnosis ( Merlini & Palladini, 2008 ). The criteria defining organ involvement in AL amyloidosis, in addition to evidence of amyloid deposits from organ or alternate-site biopsy, are summarized in Table 1 ( Gertz et al., 2005 ; National Comprehensive Cancer Network, 2018 ).

The most commonly involved organs are the kidney and the heart, either individually or together ( Grogan et al., 2017 ). In various reports of patient series or clinical studies, 48% to 80% of patients had renal involvement (Jun et al., 2013; Kyle et al., 1997 ; Merlini & Palladini, 2008 ; Michael et al., 2010 ; Skinner et al., 2004 ; Vesole et al., 2006 ), and 21% to 70% had cardiac involvement (Jun et al., 2013; Kyle et al., 1997 ; Merlini & Palladini, 2008 ; Michael et al., 2010 ; Skinner et al., 2004 ; Vesole et al., 2006 ). Recent data suggested that 80% of patients were estimated to have dominant cardiac amyloid, and two thirds had dominant renal amyloid at diagnosis ( Milani, Merlini, & Palladini, 2018 ). In patients with cardiac AL amyloidosis, advanced cardiac dysfunction is estimated to result in up to 30% of patients dying within 90 days of diagnosis ( Grogan et al., 2017 ; Palladini & Merlini, 2016 ), and AL amyloidosis patients with high FLC burden are likely to have more severe cardiac involvement ( Kumar et al., 2010 ).

Other commonly involved organs are liver and nerve systems. Approximately 16% to 22% of AL amyloidosis patients have liver involvement, manifesting as hepatomegaly and elevation of alkaline phosphatase at diagnosis ( Gertz, 2018 ; Merlini & Palladini, 2012 ; Merlini, Wechalekar, & Palladini, 2013 ), although in the presence of heart failure, the clinical differentiation of amyloid infiltration from venous congestion may not be possible ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Nervous system involvement (peripheral or autonomic) is present in 14% to 20% of patients, with 20% presenting with peripheral neuropathy ( Benson & Kincaid, 2007 ; Merlini & Palladini, 2012 ).

PRESENTING SIGNS AND SYMPTOMS

The most critical step to early and correct diagnosis is to suspect AL amyloidosis on the basis of the clinical manifestations ( Kastritis & Dimopoulos, 2016 ). A formal diagnosis of AL amyloidosis is not usually made until signs or symptoms referable to a particular organ appear ( Falk, Comenzo, & Skinner, 1997 ; Grogan et al., 2017 ; Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ; National Comprehensive Cancer Network, 2018 ). It is therefore important for advanced practice clinicians and nurses to be aware of the multiple signs and symptoms that could give rise to clinical suspicion of AL amyloidosis. Increased awareness of the potential for certain signs and symptoms to be associated with AL amyloidosis, particularly relating to the most frequently involved organs (heart and kidneys), represents an opportunity for achieving earlier diagnosis ( Lousada et al., 2015 ).

The initial presentation of AL amyloidosis can be subtle and nonspecific, and usually depends on the organ(s) involved ( Comenzo et al., 2012 ; Falk et al., 1997 ; Muchtar et al., 2017b ; National Comprehensive Cancer Network, 2018 ), such as shortness of breath, weakness, orthopnea, polyneuropathy, swelling of the ankles and legs, and macroglossia (Jun et al., 2013; Lousada et al., 2015 ; Merlini et al., 2013 ); in a retrospective analysis of Korean patients with AL amyloidosis, pitting edema was the most common initial presentation, occurring in 26% of patients ( Jun et al., 2013 ).

Cardiac involvement is common in AL amyloidosis, with cardiac amyloidosis a diagnostic consideration in patients presenting with nondilated or restrictive cardiomyopathy with preserved ejection fraction ( Carrizales-Sepulveda, Ordaz-Farias, Vera-Pineda, Benavides-Gonzalez, & Flores-Ramirez, 2017 ; Flodrova et al., 2018 ). Other signs include low-voltage QRS and pseudo infarct patterns on electrocardiogram, orthostatic hypotension associated with low cardiac output, left ventricular hypertrophy with increased interventricular septal diameter > 12 mm, and/or diastolic dysfunction, and late gadolinium enhancement on cardiac MRI. Other symptoms include shortness of breath, edema, atypical chest pain from small vessel disease, ascites, and syncope ( Carrizales-Sepulveda et al., 2017 ; Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ; Merlini & Palladini, 2012 ; Wechalekar et al., 2013 ).

Renal involvement is also common in AL amyloidosis and a diagnosis should be considered in patients presenting with nephrotic syndrome with or without renal insufficiency ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Symptoms potentially associated with AL amyloidosis with renal involvement can include ankle swelling, dizziness due to orthostatic hypotension, frothy urine from proteinuria, peripheral edema, mild renal impairment, and hypercholesterolaemia in patients with nephrotic syndrome ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). It is important to note that nephrotic range proteinuria can occur with normal renal function.

Peripheral neuropathy could be a sign of peripheral nervous system involvement in AL amyloidosis ( Benson & Kincaid, 2007 ). Specific symptoms associated with sensory neuropathy may include paresthesia, numbness, and muscle weakness due to co-occurring myopathy. Additionally, postural hypotension, impotence, dry mouth and eyes, and disturbed gastrointestinal (GI) motility may be associated with autonomic neuropathy in AL amyloidosis patients with nervous system involvement ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ; Merlini & Palladini, 2012 ; Muchtar et al., 2017b ).

Specific GI involvement may be associated with a range of presenting symptoms including weight loss, nausea, diarrhea, disturbed GI motility, and GI bleeding ( Cowan et al., 2013 ; Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ; Iida, Yamano, & Nakase, 2018 ). AL amyloidosis with hepatic involvement—another commonly involved organ site—should be considered for patients presenting with unexplained hepatomegaly with normal imaging or isolated elevated alkaline phosphatase in the absence of transaminitis ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Macroglossia, which is highly indicative of AL amyloidosis, submandibular and cervical gland enlargement, and carpal tunnel syndrome are indicative of soft tissue involvement ( Merlini & Palladini, 2012 ).

Rare Presentations

Various other symptoms, reflecting the involvement of other organ systems, may be present; indeed, any organ other than the brain can be involved in AL amyloidosis ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Approximately 15% of patients have soft tissue involvement at presentation ( Milani et al., 2018 ). Less common signs and symptoms that could indicate AL amyloidosis include: skin and soft-tissue thickening, painful seronegative arthropathy, bone involvement, hoarse voice, hypoadrenalism, hypothyroidism, lymphadenopathy, and pulmonary infiltration ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Myopathy is a rare presentation, often misdiagnosed, and serum creatine kinase levels have been shown to be an unreliable biomarker ( Muchtar et al., 2016 ). While uncommon, ocular manifestations of amyloidosis have been reported, as a result of involvement of various organ systems. The deposition of amyloid fibrils has been documented in the conjunctiva, temporal artery, extraocular muscle, trabecular meshwork, and cranial nerves ( Reynolds et al., 2017 ). Reports of temporal artery biopsies performed to confirm a diagnosis of suspected giant cell arteritis ( Ghinai et al., 2017 ; Kanaan, Lorenzi, Thampy, Pandit, & Dayan, 2017 ) and to determine the cause of bilateral sequential optic neuropathy ( Kanaan et al., 2017 ) have revealed amyloid deposits confirming a diagnosis of AL amyloidosis in these patients.

DIAGNOSTIC STRATEGIES TO CONFIRM AL AMYLOIDOSIS

A number of diagnostic workup algorithms are available in order to guide clinicians through the process of establishing a clear diagnosis of AL amyloidosis ( Gertz, 2018 ; National Comprehensive Cancer Network, 2018 ; Palladini & Merlini, 2016 ); a recently published algorithm by Gertz is reproduced in Figure 1 . If AL amyloidosis is suspected based on the presenting signs and symptoms, patients undergo an initial workup to detect the presence of amyloid deposits and to evaluate organ function ( Table 2 ). First, an assessment for the underlying plasma cell dyscrasia is performed by serum and urine protein electrophoresis and immunofixation, and the immunoglobulin FLC assay for κ and λ immunoglobulin light chains ( Merlini & Palladini, 2008 ; Palladini et al., 2017 ). If the screening tests are positive, subcutaneous fat aspiration and bone marrow biopsy are then carried out to determine the presence of amyloid by characteristic Congo red staining ( Dispenzieri et al., 2015 ; Fernandez de Larrea et al., 2015 ; Milani et al., 2018 ). Other histological staining such as Thioflavin stains may be used; however, Congo red staining is generally the most accepted and considered the standard for detection of amyloid ( Dispenzieri et al., 2015 ). Bone marrow evaluation may determine the presence of plasma cell dyscrasia, the percentage of plasma cells, and plasma cell genetics by fluorescence in situ hybridization ( Gertz, 2018 ). The salivary gland is used as an alternative site for biopsy if the abdominal fat aspirate is negative, with a sensitivity of 58% ( Foli et al., 2011 ). If fat aspirate and bone marrow are both negative but clinical suspicion for amyloid remains high, then a tissue biopsy of the organs thought to be involved (heart, liver, kidney, nerve, etc.) is collected ( Gertz, 2018 ) and stained with Congo red. Although all biopsies carry a risk of bleeding, liver biopsies should be carried out with caution due to the increased risk of hemorrhage ( Palladini & Merlini, 2016 ).

Once the diagnosis of amyloidosis has been made, the type of amyloidosis that is present must be determined. When stained using Congo red, the amyloid deposits produce a pathognomonic red-green birefringence under cross-polarized light microscopy ( Comenzo, 2007a ; Falk et al., 1997 ; Merlini & Bellotti, 2003 ). However, Congo red staining and fluorescent birefringence alone do not distinguish AL amyloidogenic protein from other forms of amyloidosis such as transthyretin (TTR), hereditary apolipoprotein AI, or acquired amyloid A (AA) amyloidosis due to chronic inflammation; typing of the amyloid deposits is therefore mandatory to confirm a diagnosis of AL amyloidosis. The most commonly available techniques to classify amyloid deposits are IHC and IF; however, these tests can be misleading when classifying amyloid deposits ( Gertz, 2018 ). A more reliable amyloid typing technique is the mass spectrometry–based proteomic analysis ( Gertz, 2018 ). Although more expensive and available to a limited number of specialized medical centers, mass spectrometry is the current gold standard for determining the type of amyloidosis ( Gertz, 2018 ). Early referral of the patient to a center of excellence where accurate amyloid typing can be done is crucial to confirm the diagnosis.

DEVELOPMENTS IN TECHNIQUES TO ASSESS ORGAN INVOLVEMENT

Once the diagnosis and typing of AL amyloidosis are established, additional evaluations may be conducted to assess the manifestation and extent of organ involvement. Patients with renal involvement commonly present with increased proteinuria (mainly albuminuria), evolving into nephrotic syndrome and progressing into renal failure if untreated ( Palladini & Merlini, 2016 ). Twenty-four-hour urinary protein loss and estimated glomerular filtration rate (eGFR) are the standard tests to evaluate renal involvement ( Palladini & Merlini, 2016 ). The assessment of proteinuria by 24 hour urine collection can be used to assess organ response and monitor organ progression. The common clinical features of liver involvement can be assessed using liver function tests to detect abnormal liver enzymes, especially elevated alkaline phosphatase, and liver imaging using CT, ultrasound, or MRI to evaluate hepatomegaly ( Palladini & Merlini, 2016 ). Suspected GI involvement may warrant endoscopic evaluation with biopsy to help confirm a diagnosis by using Congo red staining to detect the presence of amyloid ( Iida et al., 2018 ). Cardiac involvement of amyloidosis can be assessed through electrical disturbances or lowered voltage in limb leads on electrocardiography, while echocardiographic evidence of increasing wall thickness and abnormality of longitudinal ventricular strain pattern may also highlight AL amyloidosis with cardiac involvement ( Carrizales-Sepulveda et al., 2017 ; Grogan et al., 2017 ). Furthermore, specific biomarkers are used to establish and stage cardiac AL amyloidosis, including N-terminal pro-brain natriuretic peptide (NT-proBNP) and cardiac troponin T (cTnT). Cardiac MRI with gadolinium is now available to evaluate cardiac amyloidosis, particularly when echocardiogram findings are inconclusive ( Grogan et al., 2017 ). Cardiac MRI can provide specific imaging features to detect cardiac involvement by amyloidosis; however, it does not distinguish the light chain amyloidosis from non-AL type amyloidoses ( Grogan et al., 2017 ). Cardiac scintigraphy ( Gertz, 2018 ; Palladini & Merlini, 2016 ) with technetium-99m ( 99m Tc) stannous pyrophosphate (PYP) or 99m 3,3 diphosphono-1, 2-propanodicarboxylic acid (DPD) may be helpful to distinguish AL from transthyretin (ATTR) cardiac amyloidosis and potentially obviate the need for cardiac biopsy ( Gertz, 2018 ; Palladini & Merlini, 2016 ), particularly in elderly patients with the absence of a plasma cell dyscrasia ( Bokhari et al., 2013 ; Siddiqi & Ruberg, 2018 ). When a monoclonal protein is present, biopsy to detect amyloid deposit and to confirm amyloid type is required to establish an accurate diagnosis ( Grogan et al., 2017 ).

DIFFERENTIAL DIAGNOSES

The differential diagnosis for AL amyloidosis includes systemic non-AL amyloidoses (including AA and ATTR types), localized light-chain amyloidosis, and other paraprotein-associated diseases including peripheral neuropathy and immunoglobulin deposition diseases ( Guidelines Working Group of UK Myeloma Forum & British Committee for Standards in Haematology British Society for Haematology, 2004 ). Among the numerous different types of systemic amyloidosis, AL comprises 78% of new cases ( Palladini & Merlini, 2016 ). The mutated transthyretin (ATTRm) and wild-type transthyretin (ATTRwt) systemic amyloidosis are found in approximately 7% and 6% to 10% of new cases, respectively. ATTRm is hereditary and is found primarily in the peripheral and autonomic system ( Palladini & Merlini, 2016 ), the heart, and the eye; common clinical features include heart failure and peripheral or autonomic neuropathy ( Grogan et al., 2017 ). In ATTRwt the heart is involved in almost all cases, as well as the ligaments and tenosynovium ( Palladini & Merlini, 2016 ); common clinical presentations include atrial fibrillation, heart failure with carpal tunnel syndrome, tendon rupture, and spinal stenosis ( Grogan et al., 2017 ).

Distinguishing the AL and ATTR subtypes can be difficult due to the overlapping symptoms and because patients can have ATTR amyloidosis and monoclonal gammopathy of undetermined significance (MGUS; Phull et al., 2018 ). In some patients with ATTR, a monoclonal protein component and abnormal FLC have been identified, which are usually characteristic of AL amyloidosis ( Geller et al., 2017 ); for such cases, biopsy to confirm the presence of amyloid and accurate typing for distinguishing the amyloid subtypes are required. While imaging with PYP or DPD can help to distinguish ATTR from AL amyloidosis, gene sequencing should be used to determine hereditary components of amyloidosis ( Gertz, 2018 ).

Patients who are already being treated for multiple myeloma (MM) and patients with MGUS who are being monitored may also have or may develop AL amyloidosis ( Grogan et al., 2017 ; Merlini & Palladini, 2012 ). Approximately 10% to 15% of patients with MM may develop primary amyloidosis and up to 38% of patients with MM have been reported to have AL amyloid deposits in the absence of readily discernible signs or symptoms ( Desikan et al., 1997 ; Madan et al., 2010 ; Rajkumar, Gertz, & Kyle, 1998 ). Due to the clonal nature of all three conditions, patients with MM or MGUS should be assessed carefully for symptoms specific to AL amyloidosis in order to establish a diagnosis as early as possible ( Merlini & Palladini, 2012 ). Assessment for an abnormal FLC ratio and for abnormal cardiac or renal parameters can help begin the diagnostic process prior to symptomatic manifestation of organ involvement in patients with MGUS ( Merlini & Palladini, 2012 ); additional “red flags” have been suggested for early identification of AL amyloidosis with other organ involvement, such as alkaline phosphatase elevation, neuropathic pain, and the onset of hypotension ( Merlini & Palladini, 2012 ). In patients with AL amyloidosis secondary to MM, specific non-myeloma symptoms require investigation; for example, one case report in the literature highlighted a female patient with MM who also presented with macroglossia and restricted tongue movement ( Dawoud & Ariyaratnam, 2016 ). Diagnosis of AL amyloidosis was established by the identification of amyloid deposits in the oral cavity following a full oral soft-tissue examination ( Dawoud & Ariyaratnam, 2016 ).

STAGING OF AL AMYLOIDOSIS

Following confirmation of AL amyloidosis, disease stage is determined. The survival outcome of patients with AL amyloidosis varies greatly and often depends on the severity of organ involvement, in particular cardiac dysfunction at diagnosis. Staging of AL amyloidosis at diagnosis allows prognostic stratification in order to select the optimal treatment approach in which the intensity is balanced with the patients’ performance status ( Milani et al., 2018 ). Patients with advanced cardiac disease with no treatment (Mayo stages IIIa and IIIb) had a median survival of less than 1 year ( Palladini, Milani, & Merlini, 2015 ). Current validated staging systems are outlined in Table 3 . The original Mayo Clinic 2004 prognostic staging system uses serum cTnT (< 0.035 ng/mL) and NT-proBNP (< 332 pg/mL) to stratify AL amyloidosis into three stages ( Dispenzieri et al., 2004 ). The revised Mayo Clinic staging includes the difference between involved and uninvolved FLC (dFLC) as prognostic variables, where one point is assigned for each of dFLC ≥ 18 mg/dL, cTnT ≥ 0.025 ng/mL, or NT-proBNP ≥ 1,800 pg/mL ( Kumar et al., 2012 ). The European staging system further added systolic blood pressure < 100 mm Hg and NT-proBNP > 8,500 pg/mL to further classify the stage III from the original Mayo 2004 staging system into stage IIIa and IIIb ( Wechalekar et al., 2013 ).

SENSITIVITY AND DETECTION RATES OF DIAGNOSTIC TESTS

Serum and urine immunofixation electrophoresis have been shown to detect a monoclonal component (with λ/κ ratio of 75/25) in 97% of patients, and FLC measurements have been shown to have a sensitivity of 76% ( Merlini & Palladini, 2008 ), while other studies have demonstrated higher detection rates (up to 97%) by FLC assay in AL amyloidosis patients ( Bochtler et al., 2008 ; Morris et al., 2007 ). Subcutaneous fat aspirate is the least invasive and often the first approach, offering 79% sensitivity in AL amyloidosis ( Fernandez de Larrea et al., 2015 ). However, target organ biopsy should be pursued if clinical suspicion remains high and fat aspirate is negative, as a biopsy sample from a symptomatic organ has higher sensitivity than that from the more accessible tissues ( Dispenzieri et al., 2015 ). Combined with bone marrow biopsy, subcutaneous fat aspirate can demonstrate AL amyloidosis in more than 85% of patients ( Gertz, 2018 ), while other studies have demonstrated a sensitivity of 98.9% for AL amyloidosis with renal involvement through a combination of skin, fat, and rectal mucosal biopsies ( Li et al., 2017 ), and of 84% for AL amyloidosis with cardiac involvement using abdominal fat pad fine-needle aspiration ( Quarta et al., 2017 ). Further, a recent analysis showed that the sensitivity of abdominal fat pad excisional biopsy could be increased to almost 100% if large enough biopsies (> 700 mm 3 ) were used ( Garcia, Collins, & Stone, 2018 ). It has been reported that fat aspirate is being underused for diagnosis of AL amyloidosis, and that a substantial proportion of patients could avoid a more invasive organ biopsy due to the sensitivity and utility of fat pad aspiration for recognizing the disease ( Muchtar et al., 2017a ).

CONCLUSIONS

Early recognition of signs and symptoms potentially associated with AL amyloidosis is the key to identification and diagnosis of the disease before substantial organ damage can occur and is thus important for improving treatment outcomes and survival. Advanced practice clinicians and oncology nurses should be aware of the common presenting signs and symptoms that could give rise to clinical suspicion of AL amyloidosis with specific organ involvement. They should also be cognizant of the subsequent needs for further diagnostic workup and, if warranted, urgent referral to a center of excellence. This would ensure an early diagnosis of AL amyloidosis when therapeutic treatments are more effective, and subsequently result in improved prognosis and quality of life of patients. Patients with AL amyloidosis should ultimately be evaluated by a team of amyloid experts to help guide the treatment choices.

Acknowledgment

The authors would like to thank Steve Hill and Laura Webb of FireKite (an Ashfield Company, part of UDG Healthcare PLC) for medical writing assistance, which was funded by Millennium Pharmaceuticals, Inc. All editorial procedures complied with Good Publication Practice-3 (GPP3) guidelines ( Battisti et al., 2015 ).

Yi L. Hwa, Teresa Fogaren, Allison Sams, and Lisa Mendelson have nothing to disclose. Douglas V. Faller, Dawn M. Stull, and Sara Thuenemann are employees of Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts, a wholly owned subsidiary of Takeda Pharmaceutical Company Limited.

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