Neutralization Tests for Quantification of Specific Antibodies Against Yellow Fever Virus.

The plaque reduction neutralization test (PRNT) is considered the reference for the analysis of humoral immune response to infection and to vaccination against the yellow fever virus, showing superior accuracy (sensitivity and specificity) to the other serological tests. The micro-PRNT version (in 96-well plates) is more operational and has a higher sample throughput than the classic PRNT run in 6-well plates. Here we described both methods to quantify yellow fever neutralizing antibodies.