Nature communications · 2023
Dynamic chromatin architecture of the porcine adipose tissues with weight gain and loss.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Jin Long, Wang Danyang, Zhang Jiaman, Liu Pengliang, Wang Yujie, Lin Yu, Liu Can, Han Ziyin, Long Keren, Li Diyan, Jiang Yu, Li Guisen, Zhang Yu, Bai Jingyi, Li Xiaokai, Li Jing, Lu Lu, Kong Fanli, Wang Xun, Li Hua, Huang Zhiqing, Ma Jideng, Fan Xiaolan, Shen Linyuan, Zhu Li, Jiang Yanzhi, Tang Guoqing, Feng Bin, Zeng Bo, Ge Liangpeng, Li Xuewei, Tang Qianzi, Zhang Zhihua, Li Mingzhou
Аннотация
Using an adult female miniature pig model with diet-induced weight gain/weight loss, we investigated the regulatory mechanisms of three-dimensional (3D) genome architecture in adipose tissues (ATs) associated with obesity. We generated 249 high-resolution in situ Hi-C chromatin contact maps of subcutaneous AT and three visceral ATs, analyzing transcriptomic and chromatin architectural changes under different nutritional treatments. We find that chromatin architecture remodeling underpins transcriptomic divergence in ATs, potentially linked to metabolic risks in obesity development. Analysis of chromatin architecture among subcutaneous ATs of different mammals suggests the presence of transcriptional regulatory divergence that could explain phenotypic, physiological, and functional differences in ATs. Regulatory element conservation analysis in pigs and humans reveals similarities in the regulatory circuitry of genes responsible for the obesity phenotype and identified non-conserved elements in species-specific gene sets that underpin AT specialization. This work provides a data-rich tool for discovering obesity-related regulatory elements in humans and pigs.