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DOI 10.3390/v16010023

Медицинская библиотека · Viruses · 2023

A Multiplex Nanopore Sequencing Approach for the Detection of Multiple Arboviral Species.

Xavier Joilson, Fonseca Vagner, Adelino Talita, Iani Felipe C M, Pereira Glauco C, Duarte Myrian M, Lima Mauricio, Castro Emerson, Oliveira Carla, Fritsch Hegger, Guimarães Natalia, Lamounier Ludmila O, Barreto Fernanda Khouri, Braga de Oliveira Camilo M M, Maymone Gonçalves Crhistinne C, Malta Lima Danielle, de Oliveira Elaine C, de Castro Lichs Gislene G, Gomes Iago, Mazaro Janaina, Rodrigues Janete T N, Abrantes Jayra, Colares Jeová K B, Luz Kleber G, Barbosa da Silva Luana, Demarchi Luiz, Câmara Magaly C B, Umaki Zardin Marina C S, Sabatini Mello Pinheiro Rafaela, Barbosa Souza Rutilene, Haddad Simone K, Figueiredo da Silva Stephanni, Slavov Svetoslav N, Rocha Themis, Morel Noelia, Chiparelli Hector, Burgueño Analía, Bórmida Victoria, Cortinas María N, Martín Rosario S, Pereira Allan C, Dos Santos Marcelo F, André Júnior Walter, Mendez Rico Jairo, Franco Leticia, Rosewell Alexander, do Carmo Said Rodrigo F, de Albuquerque Carlos F C, Noia Maciel Ethel L, Santini de Oliveira Marília, Venâncio da Cunha Rivaldo, Vinhal Frutuoso Livia C, de Filippis Ana M B, Giovanetti Marta, Carlos Junior Alcantara Luiz

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Аннотация

The emergence and continued geographic expansion of arboviruses and the growing number of infected people have highlighted the need to develop and improve multiplex methods for rapid and specific detection of pathogens. Sequencing technologies are promising tools that can help in the laboratory diagnosis of conditions that share common symptoms, such as pathologies caused by emerging arboviruses. In this study, we integrated nanopore sequencing and the advantages of reverse transcription polymerase chain reaction (RT-PCR) to develop a multiplex RT-PCR protocol for the detection of Chikungunya virus (CHIKV) and several orthoflaviviruses (such as dengue (Orthoflavivirus dengue), Zika (Orthoflavivirus zikaense), yellow fever (Orthoflavivirus flavi), and West Nile (Orthoflavivirus nilense) viruses) in a single reaction, which provides data for sequence-based differentiation of arbovirus lineages.

Для цитирования: Xavier Joilson, Fonseca Vagner, Adelino Talita, Iani Felipe C M, Pereira Glauco C, Duarte Myrian M, Lima Mauricio, Castro Emerson, Oliveira Carla, Fritsch Hegger, Guimarães Natalia, Lamounier Ludmila O, Barreto Fernanda Khouri, Braga de Oliveira Camilo M M, Maymone Gonçalves Crhistinne C, Malta Lima Danielle, de Oliveira Elaine C, de Castro Lichs Gislene G, Gomes Iago, Mazaro Janaina, Rodrigues Janete T N, Abrantes Jayra, Colares Jeová K B, Luz Kleber G, Barbosa da Silva Luana, Demarchi Luiz, Câmara Magaly C B, Umaki Zardin Marina C S, Sabatini Mello Pinheiro Rafaela, Barbosa Souza Rutilene, Haddad Simone K, Figueiredo da Silva Stephanni, Slavov Svetoslav N, Rocha Themis, Morel Noelia, Chiparelli Hector, Burgueño Analía, Bórmida Victoria, Cortinas María N, Martín Rosario S, Pereira Allan C, Dos Santos Marcelo F, André Júnior Walter, Mendez Rico Jairo, Franco Leticia, Rosewell Alexander, do Carmo Said Rodrigo F, de Albuquerque Carlos F C, Noia Maciel Ethel L, Santini de Oliveira Marília, Venâncio da Cunha Rivaldo, Vinhal Frutuoso Livia C, de Filippis Ana M B, Giovanetti Marta, Carlos Junior Alcantara Luiz. A Multiplex Nanopore Sequencing Approach for the Detection of Multiple Arboviral Species.. Viruses. 2023. DOI: 10.3390/v16010023.

Предметные рубрики MeSH

HumansArbovirusesNanopore SequencingChikungunya virusMultiplex Polymerase Chain ReactionZika VirusZika Virus InfectionDengue
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