Analytica chimica acta · 2026
Highly sensitive chemiluminescence immunoassay utilizing functionalized magnetic metal-organic framework materials for the detection of CEA and CA199.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zuo Xiaomin, Chen Wenxia, Lu Xianlong, Yuan Rui, Hu Shuangyi, Xue Yangyang, Chen Wei, Yang Rui
Аннотация
BACKGROUND: In response to the urgent need for highly sensitive and rapid combined detection of multiple tumor markers for the early diagnosis of colorectal cancer, this work innovatively constructs a magnetically-driven chemiluminescence (CL) immunosensing platform (CoFe2O4@ZIF-8/luminol-Au NPs).
RESULTS: CoFe2O4 NPs were firstly aminated via solvothermal method and lysine modification to provide specific binding sites. ZIF-8 was then grown in situ to form a core-shell structure with high specific surface area for efficient luminol loading. Finally, Au NPs were generated in situ within ZIF-8, simultaneously immobilizing luminol to form composite signal units. This design integrates magnetic separation, confinement effects, and signal amplification, generating an ideal microenvironment for enhanced CL reactions. The label-free CL immunoassay achieves an impressive limit of detection (LOD) of 22 fg/mL for CEA and 5.4 fg/mL for CA199, with broad linear ranges spanning over 5 orders of magnitude. The entire detection process is completed within 30 min, and the analysis of real serum samples demonstrates good recoveries.
RESULTS: CoFe2O4 NPs were firstly aminated via solvothermal method and lysine modification to provide specific binding sites. ZIF-8 was then grown in situ to form a core-shell structure with high specific surface area for efficient luminol loading. Finally, Au NPs were generated in situ within ZIF-8, simultaneously immobilizing luminol to form composite signal units. This design integrates magnetic separation, confinement effects, and signal amplification, generating an ideal microenvironment for enhanced CL reactions. The label-free CL immunoassay achieves an impressive limit of detection (LOD) of 22 fg/mL for CEA and 5.4 fg/mL for CA199, with broad linear ranges spanning over 5 orders of magnitude. The entire detection process is completed within 30 min, and the analysis of real serum samples demonstrates good recoveries.