Scientific reports · 2019
Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016-2017).
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Cunha Mariana Sequetin, da Costa Antonio Charlys, de Azevedo Fernandes Natália Coelho Couto, Guerra Juliana Mariotti, Dos Santos Fabiana Cristina Pereira, Nogueira Juliana Silva, D'Agostino Leandro Guariglia, Komninakis Shirley Vasconcelos, Witkin Steven S, Ressio Rodrigo Albergaria, Maeda Adriana Yurika, Vasami Fernanda Gisele Silva, Kaigawa Ursula Mitsue Abreu, de Azevedo Laís Sampaio, de Souza Facioli Paloma Alana, Macedo Fernando Luiz Lima, Sabino Ester Cerdeira, Leal Élcio, de Souza Renato Pereira
Аннотация
Beginning in late 2016 Brazil faced the worst outbreak of Yellow Fever in recent decades, mainly located in southeastern rural regions of the country. In the present study we characterize the Yellow Fever Virus (YFV) associated with this outbreak in São Paulo State, Brazil. Blood or tissues collected from 430 dead monkeys and 1030 pools containing a total of 5,518 mosquitoes were tested for YFV by quantitative RT-PCR, immunohistochemistry (IHC) and indirect immunofluorescence. A total of 67 monkeys were YFV-positive and 3 pools yielded YFV following culture in a C6/36 cell line. Analysis of five nearly full length genomes of YFV from collected samples was consistent with evidence that the virus associated with the São Paulo outbreak originated in Minas Gerais. The phylogenetic analysis also showed that strains involved in the 2016-2017 outbreak in distinct Brazilian states (i.e., Minas Gerais, Rio de Janeiro, Espirito Santo) intermingled in maximum-likelihood and Bayesian trees. Conversely, the strains detected in São Paulo formed a monophyletic cluster, suggesting that they were local-adapted. The finding of YFV by RT-PCR in five Callithrix monkeys who were all YFV-negative by histopathology or immunohistochemistry suggests that this YFV lineage circulating in Sao Paulo is associated with different outcomes in Callithrix when compared to other monkeys.