Oral health & preventive dentistry · 2026
Casearia sylvestris Derivatives Inhibit Inflammation and Oxidative Activity and Promote Healing in Human Gingival Fibroblasts - An In Vitro Study.
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Pavanelli Angélica Letícia Reis, Lopes Maria Eduarda Scordamaia, Reis André Thiago, Carvalho Flávio Alexandre, Zalewski Sven, Dos Santos André Gonzaga, Eick Sigrun, Cirelli Joni Augusto, Nogueira Andressa Vilas Boas, Deschner James
Аннотация
PURPOSE: New promising adjuvant approaches for managing periodontal inflammation are emerging. Casearia sylvestris, a medicinal plant rich in diterpenes, has demonstrated anti-inflammatory, antioxidant, and reparative properties. The present study aimed to investigate the anti-inflammatory, antiproteolytic, and pro-healing effects of C. sylvestris leaf derivatives: ethanolic extract (extract), diterpene-enriched fraction (F2), and isolated casearin J (Cas J) on human gingival fibroblasts (HGF).
METHODS AND MATERIALS: HGF were exposed to Escherichia coli lipopolysaccharide (LPS) and treated with C. sylvestris derivatives. Transcription and protein levels of TNF-α, IL-8, MMP-1, and iNOS were assessed by real-time polymerase chain reaction (PCR) and ELISA, respectively. Nitric oxide (NO) levels were measured using the Griess reaction. NF-κB p65 nuclear translocation and ERK1 phosphorylation were analysed by immunofluorescence and immunoassay, respectively. Cell viability and scratch wound-healing assays were also performed.
RESULTS: E. coli LPS triggered a significant pro-inflammatory and proteolytic cellular response, characterised by increased TNF-α, IL-8, MMP-1, iNOS, and NO levels. Treatment with C. sylvestris derivatives significantly reduced the levels of these mediators, while also inhibiting NF-κB p65 translocation and ERK1 phosphorylation. All compounds were biocompatible and promoted cell migration and wound closure in vitro.
CONCLUSION: These findings suggest that C. sylvestris leaf derivatives exert anti-inflammatory, antiproteolytic, and pro-healing effects, thus highlighting their potential as adjunctive phytotherapeutic agents for periodontal disease management.
METHODS AND MATERIALS: HGF were exposed to Escherichia coli lipopolysaccharide (LPS) and treated with C. sylvestris derivatives. Transcription and protein levels of TNF-α, IL-8, MMP-1, and iNOS were assessed by real-time polymerase chain reaction (PCR) and ELISA, respectively. Nitric oxide (NO) levels were measured using the Griess reaction. NF-κB p65 nuclear translocation and ERK1 phosphorylation were analysed by immunofluorescence and immunoassay, respectively. Cell viability and scratch wound-healing assays were also performed.
RESULTS: E. coli LPS triggered a significant pro-inflammatory and proteolytic cellular response, characterised by increased TNF-α, IL-8, MMP-1, iNOS, and NO levels. Treatment with C. sylvestris derivatives significantly reduced the levels of these mediators, while also inhibiting NF-κB p65 translocation and ERK1 phosphorylation. All compounds were biocompatible and promoted cell migration and wound closure in vitro.
CONCLUSION: These findings suggest that C. sylvestris leaf derivatives exert anti-inflammatory, antiproteolytic, and pro-healing effects, thus highlighting their potential as adjunctive phytotherapeutic agents for periodontal disease management.