Chromosome-level genome assembly of Nekemias grossedentata provides new insights into genome evolution and flavonoid biosynthesis.

•A chromosome-scale genome assembly of Nmpelopsis grossedentata provides a foundational genomic resource.•Karyotype analysis positions N. grossedentata as having a karyotype most closely resembling the Ancestral Eudicot Karyotype (AEK) than Muscadinia rotundifolia or V itis vinifera.•This study offers key insights into the genomic basis of flavonoid biosynthesis and karyotype evolution in plants.