Grass Expression Atlas: an RNA-seq-based expression resource for grass species.

Grass Expression Atlas (GExA) is an interactive web-based resource for rapid exploration of gene expression across diverse tissues, developmental stages, and conditions in grass species. GExA integrates publicly available RNA sequencing (RNA-seq) datasets for four millets: pearl millet (Cenchrus americanus), foxtail millet (Setaria italica), proso millet (Panicum miliaceum), and finger millet (Eleusine coracana), and includes barley (Hordeum vulgare), sorghum (Sorghum bicolor) and rice (Oryza sativa) as reference species. Public RNA-seq datasets were reanalyzed using a unified workflow within each species to obtain read counts for each gene, which were then converted into consistently formatted expression matrices in transcripts per million (TPM). The current release comprises 4,753 samples from 457 BioProjects, including 987 pearl millet samples and 2,216 foxtail millet samples, and is provided through a user-friendly web interface. GExA is designed for scalable expansion to additional species via the pipeline used in this study. GExA is freely available athttps://www.gexa.anesc.u-tokyo.ac.jp/.