A role for spinal lamina I neurokinin-1-positive neurons in cold thermoreception in the rat.

Lamina I neurons of the spinal cord convey specific nociceptive activity to the brain. A subpopulation of lamina I cells bears substance P receptors (neurokinin-1) and recent studies have shown that these neurons encode for the intensity of noxious peripheral stimulation. Here, we report that cool thermal stimuli, applied to the hindpaw of anaesthetized rats, induce Fos expression in lamina I neurokinin-1 neurons that is graded with respect to the intensity of the thermal stimulus. Thus, as the temperature of the stimulus was reduced, both the total number of neurokinin-l-positive neurons expressing Fos and the proportion of Fos nuclei present within neurokinin-1 cells showed a significant increase. These data show that lamina I neurokinin-1 cells encode the intensity of noxious cooling of the skin. In laminae III and IV, although there was no correlation between neurokinin-1 cell activation and stimulus intensity, the total Fos count in these layers was inversely related to the depth of cooling. Thus, neurons in laminae III and IV may also play a role in thermoreception.