LIM domain-containing proteins Zyxin and LPP localize to apical epithelial cell-cell junctions at regions of stable F-actin.

LIM domain-containing proteins, Zyxin and Lipoma-Preferred Partner (LPP), localize to sites where actin filaments are strained, including actin stress fibers, focal adhesions, and cell-cell junctions. Utilizing the Xenopus laevis embryonic epithelium and live confocal microscopy, this study demonstrates that Zyxin and LPP localize to apical cell-cell junctions in polarized epithelia. Furthermore, particle image velocimetry (PIV) analysis reveals an inverse correlation between Zyxin/LPP intensity and F-actin velocity, suggesting that Zyxin and LPP are present at regions of stable F-actin.