Revista do Instituto de Medicina Tropical de Sao Paulo · 2011
Isolation of yellow fever virus (YFV) from naturally infected Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Souza Renato Pereira de, Petrella Selma, Coimbra Terezinha Lisieux Moraes, Maeda Adriana Yurika, Rocco Iray Maria, Bisordi Ivani, Silveira Vivian Regina, Pereira Luiz Eloy, Suzuki Akemi, Silva Sarai Joaquim Dos Santos, Silva Fernanda Gisele, Salvador Felipe Scassi, Tubaki Rosa Maria, Menezes Regiane Tironi, Pereira Mariza, Bergo Eduardo Sterlino, Hoffmann Roberto Colozza, Spinola Roberta Maria Fernandes, Tengan Cílea Hatsumi, Siciliano Melissa Mascheratti
Аннотация
After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus.