Responsible biotechnology: an analysis of the Asilomar 2025 entreaties.

Fifty years after the original Asilomar Meeting on Recombinant DNA, new stakeholders in biotechnology reconvened at the Spirit of Asilomar and Future of Biotechnology 2025 Conference. The objective was the responsible advancement of biotechnology, with open-ended themes converging around the environmental release of engineered organisms, biosecurity, artificial intelligence in biological sciences, education, governance, regulation, and community practice. This meeting led to 27 published documents ('entreaties'). Here, we synthesize them into action items and contextualize the guidance within the broader literature. The entreaty corpus encompasses different viewpoints that reflect the need to revisit these sometimes-half-century-old topics in light of modern and future advances in biotechnology.