International journal of nanomedicine · 2018
A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells.
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Zhu Jianmeng, Li Yiping, Li Lei, Wang Jian, Wang Hongqin, Hong Wenzhong, Hao Ke, Xue Yadan, Chen Bingyu, Wang Zhen
Аннотация
INTRODUCTION: Detection of antigen-specific cytotoxic T lymphocytes (CTLs) is the foundation for understanding hepatocellular carcinoma immune pathology and hepatocellular carcinoma immunotherapy. However, the classical method for labeling CTLs, major histocompatibility complex (MHC)-peptide tetramer, has drawbacks and needs further improvement.
MATERIALS AND METHODS: Here, as a new detection probe, a graphene-based MHC-peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO-streptavidin complex. Biotinylated MHC-peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine.
RESULTS: Using HRGO/MHC-peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients.
CONCLUSION: HRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells.
MATERIALS AND METHODS: Here, as a new detection probe, a graphene-based MHC-peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO-streptavidin complex. Biotinylated MHC-peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine.
RESULTS: Using HRGO/MHC-peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients.
CONCLUSION: HRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells.