Investigative ophthalmology & visual science · 2010
Complement factor h autoantibodies and age-related macular degeneration.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Dhillon Baljean, Wright Alan F, Tufail Adnan, Pappworth Isabel, Hayward Caroline, Moore Iain, Strain Lisa, Kavanagh David, Barlow Paul N, Herbert Andrew P, Schmidt Christoph Q, Armbrecht Ana-Maria, Laude Augustinus, Deary Ian J, Staniforth Scott J, Holmes Lucy V, Goodship Timothy H J, Marchbank Kevin J
Аннотация
PURPOSE: In this case-control study, the hypothesis that factor H autoantibodies are associated with age-related macular degeneration (AMD) was examined.
METHODS: One hundred AMD patients (median age, 78 years), 98 age-matched control subjects (median age, 78 years) known not to have AMD, and 100 healthy blood donors (median age, 43 years) were enrolled. An enzyme-linked immunosorbent assay (ELISA) was used to screen for complement factor H autoantibodies and either quantitative polymerase chain reaction (qPCR) or multiplex ligation-dependent probe amplification (MLPA) were performed to measure the copy number of the gene encoding complement factor H-related protein 3 (CFHR3).
RESULTS: There was a significant difference in the median complement factor H autoantibody titer between the three groups (AMD patients, 196 reference units [RU]]; age-match control subjects, 316 RU; and blood donor control subjects, 121 RU; Kruskal-Wallis test, P < 0.001). Pair-wise comparison (Mann-Whitney test) showed that all three groups were significantly different from each other. Two different thresholds were used in the healthy blood donors to identify individuals with complement factor H autoantibodies. Both suggested that the prevalence of factor H autoantibodies was decreased in AMD patients. The CFHR3 copy number was measured as a surrogate for the deletion of the genes encoding complement factor H-related proteins 3 and 1 (CFHR3/1). The allele frequency of the deletion was significantly higher in the age-matched control subjects than in the AMD patients (22.2% vs. 8.2%).
CONCLUSIONS: The level of factor H autoantibodies is lower in AMD patients than in age-matched control subjects.
METHODS: One hundred AMD patients (median age, 78 years), 98 age-matched control subjects (median age, 78 years) known not to have AMD, and 100 healthy blood donors (median age, 43 years) were enrolled. An enzyme-linked immunosorbent assay (ELISA) was used to screen for complement factor H autoantibodies and either quantitative polymerase chain reaction (qPCR) or multiplex ligation-dependent probe amplification (MLPA) were performed to measure the copy number of the gene encoding complement factor H-related protein 3 (CFHR3).
RESULTS: There was a significant difference in the median complement factor H autoantibody titer between the three groups (AMD patients, 196 reference units [RU]]; age-match control subjects, 316 RU; and blood donor control subjects, 121 RU; Kruskal-Wallis test, P < 0.001). Pair-wise comparison (Mann-Whitney test) showed that all three groups were significantly different from each other. Two different thresholds were used in the healthy blood donors to identify individuals with complement factor H autoantibodies. Both suggested that the prevalence of factor H autoantibodies was decreased in AMD patients. The CFHR3 copy number was measured as a surrogate for the deletion of the genes encoding complement factor H-related proteins 3 and 1 (CFHR3/1). The allele frequency of the deletion was significantly higher in the age-matched control subjects than in the AMD patients (22.2% vs. 8.2%).
CONCLUSIONS: The level of factor H autoantibodies is lower in AMD patients than in age-matched control subjects.
Предметные рубрики MeSH
AdolescentAdultAgedAged, 80 and overAutoantibodiesBlood DonorsBlood ProteinsCase-Control StudiesComplement C3b Inactivator ProteinsComplement Factor HDNA PrimersDNA ProbesEnzyme-Linked Immunosorbent AssayFemaleGene DosageGene FrequencyHumansMacular DegenerationMaleMiddle AgedNucleic Acid Amplification TechniquesPolymerase Chain ReactionYoung Adult