Sao Paulo medical journal = Revista paulista de medicina · 1999
Evaluation of three methods for hemoglobin measurement in a blood donor setting.
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Rosenblit J, Abreu C R, Szterling L N, Kutner J M, Hamerschlak N, Frutuoso P, Paiva T R, Ferreira O C
Аннотация
CONTEXT: The hemoglobin (Hb) level is the most-used parameter for screening blood donors for the presence of anemia, one of the most-used methods for measuring Hb levels is based on photometric detection of cyanmetahemoglobin, as an alternative to this technology, HemoCue has developed a photometric method based on the determination of azide metahemoglobin.
OBJECTIVE: To evaluate the performance of three methods for hemoglobin (Hb) determination in a blood bank setting.
DESIGN: Prospective study utilizing blood samples to compare methods for Hb determination.
SETTING: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system.
SAMPLE: Serial blood samples were collected from 259 individuals during the period from March to June 1996.
MAIN MEASUREMENTS: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV), linear regression and mean differences.
RESULTS: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05). The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (+/- 0.78 and +/- 1.02) than the Coulter and Cobas combination (+/- 1.13).
CONCLUSION: The three methods provide good agreement for hemoglobin determination.
OBJECTIVE: To evaluate the performance of three methods for hemoglobin (Hb) determination in a blood bank setting.
DESIGN: Prospective study utilizing blood samples to compare methods for Hb determination.
SETTING: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system.
SAMPLE: Serial blood samples were collected from 259 individuals during the period from March to June 1996.
MAIN MEASUREMENTS: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV), linear regression and mean differences.
RESULTS: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05). The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (+/- 0.78 and +/- 1.02) than the Coulter and Cobas combination (+/- 1.13).
CONCLUSION: The three methods provide good agreement for hemoglobin determination.