Transfusion · 2026
Emergency whole-tank transfer of cord blood units after cryogenic failure: A safe rescue strategy.
Клинический случайСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fernandez-Sojo Jesus, Azqueta Carmen, Castillo-Flores Nerea, Linares Mònica, Valdivia Elena, Medina-Boronat Laura, Arteaga Sergio, Rubio Nuria, Poyatos Sonia, Guterres Alexander, Garcia-Buendia Ana, Querol Sergi, Grifols Joan R
Аннотация
BACKGROUND: Cryogenic storage of cord blood units (CBUs) is critical to ensure long-term viability and safety. Loss of vacuum in liquid nitrogen (LN2) tanks is an exceptionally rare but high-risk event that may lead to accelerated LN2 evaporation and potential warming of stored units.
CASE REPORT: We describe an emergency rescue transfer following suspected vacuum loss in a BioArchive cryogenic tank at a public cord blood bank. The tank contained 2880 CBUs, including 14 directed units. Progressive LN2 consumption suggested structural failure, and in situ repair was not feasible. A rapid transfer procedure using a backup BioArchive-compatible tank was performed with the assistance of the manufacturer. The maneuver involved crane-assisted repositioning of the tank under the robotic system and was completed in 40 s. Continuous temperature monitoring confirmed stable vapor-phase conditions throughout the procedure. Six CBUs stored in upper tank positions (worst-case exposure) were evaluated post-thaw. Cell recovery, viability, and clonogenic capacity were preserved and met release criteria for transplantation.
CONCLUSION: Emergency transfer of CBUs during cryogenic tank malfunction is feasible and can be performed safely without compromising product quality when executed under controlled conditions. This report provides practical guidance for managing rare but critical storage failures in cord blood banking using BioArchive system.
CASE REPORT: We describe an emergency rescue transfer following suspected vacuum loss in a BioArchive cryogenic tank at a public cord blood bank. The tank contained 2880 CBUs, including 14 directed units. Progressive LN2 consumption suggested structural failure, and in situ repair was not feasible. A rapid transfer procedure using a backup BioArchive-compatible tank was performed with the assistance of the manufacturer. The maneuver involved crane-assisted repositioning of the tank under the robotic system and was completed in 40 s. Continuous temperature monitoring confirmed stable vapor-phase conditions throughout the procedure. Six CBUs stored in upper tank positions (worst-case exposure) were evaluated post-thaw. Cell recovery, viability, and clonogenic capacity were preserved and met release criteria for transplantation.
CONCLUSION: Emergency transfer of CBUs during cryogenic tank malfunction is feasible and can be performed safely without compromising product quality when executed under controlled conditions. This report provides practical guidance for managing rare but critical storage failures in cord blood banking using BioArchive system.