[Transplantation immunology in allogeneic bone marrow transplantation. The significance of HLA-tissue crossmatching between donors and recipients].

We describe our current programme for donor-patient HLA matching. First, the patient and family members are serologically typed to define HLA haplotypes and to search for HLA genotypically identical siblings. If no HLA identical siblings can be found, we search for a haploidentical family donor with zero or at most one HLA molecule mismatch on the non-shared haplotype. Secondly, we search for an HLA identical unrelated donor in the Norwegian and foreign bone marrow donor registers. For final matching of patient and unrelated or haploidentical donors, we include genomic typing of DRB1, DQB1, and DPB1 alleles by sequence-specific oligonucleotide probing or direct sequencing.