International journal of antimicrobial agents · 2025
Genetic characterization of extensively drug-resistant blaCTX-M-27Shigella sonnei clusters among men who have sex with men in a region of northern Spain.
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Vecilla Domingo Fernández, Gutiérrez Mikel Joseba Urrutikoetxea, Toboso María Carmen Nieto, Inchaurza Kristina Zugazaga, Zárraga Estíbaliz Ugalde, Estévez Beatriz Ruiz, de Tuesta Del Arco José Luis Díaz
Аннотация
OBJECTIVE: The convergence of globalization with increased sexual risk behaviours has significantly facilitated the dissemination of multidrug-resistant and extensively drug-resistant clusters of Shigella spp. among men who have sex with men, particularly Shigella sonnei and Shigella flexneri. A cluster of S. sonnei carrying blaCTX-M-27 caused a European outbreak in 2020-2021, with more than 30 cases in Spain, including two in our institution. In this study, we conducted a retrospective study from October 2022 to December 2023 that included five additional patients with shigellosis caused by a CTX-M-27-producing S. sonnei.
METHODS: Genetic characterization was assessed by whole-genome sequencing using the MinION Mk1C device (Oxford Nanopore Technologies, Oxford, UK).
RESULTS: All the isolates presented IncB/O/K/Z or IncFII plasmids, which carried genes conferring resistance to second- and third-generation cephalosporins, cotrimoxazole, azithromycin and quinolones. SNP analysis revealed that neither the strains within this study nor the UK cluster were related to each other.
CONCLUSIONS: Different community clusters of extensively drug-resistant S. sonnei strains harbouring blaCTX-M-27 are spreading in our area, mainly associated with sexual transmission among men who have sex with men.
METHODS: Genetic characterization was assessed by whole-genome sequencing using the MinION Mk1C device (Oxford Nanopore Technologies, Oxford, UK).
RESULTS: All the isolates presented IncB/O/K/Z or IncFII plasmids, which carried genes conferring resistance to second- and third-generation cephalosporins, cotrimoxazole, azithromycin and quinolones. SNP analysis revealed that neither the strains within this study nor the UK cluster were related to each other.
CONCLUSIONS: Different community clusters of extensively drug-resistant S. sonnei strains harbouring blaCTX-M-27 are spreading in our area, mainly associated with sexual transmission among men who have sex with men.