Cx3cr1 -BAC-CRE-mediated knockout of toll-like receptor 4 alters mouse communicative behaviors, microglial morphology, and engulfment of synaptic material.

Microglia, the tissue resident immune cells of the central nervous system, are critical regulators of postnatal neural circuit refinement. Innate immune receptors on microglia such as toll-like receptor 4 (TLR4) are best characterized in the context of inflammation, however, endogenous TLR4 ligands are generated during typical developmental processes. Here, we investigated whether TLR4 signaling in CX3CR1-expressing myeloid cells contributes to microglial morphology, synaptic engulfment, and behavioral development in mice under basal conditions. TLR4 conditional knockout altered maternal separation-induced ultrasonic vocalizations without impacting social preference or anxiety-like behaviors. Conditional TLR4 knockout in CX3CR1-expressing myeloid cells markedly increased microglial ramification and cell volume within the paraventricular nucleus (PVN) of the hypothalamus. TLR4 conditional knockout also reduced microglial engulfment of vGlut2-positive presynaptic material, while overall excitatory synapse numbers remained unchanged. Together, these findings demonstrate that TLR4 signaling in CX3CR1-expressing myeloid cells contributes to microglial morphology, presynaptic engulfment, and neonatal communicative behavior under basal conditions.