CRANBERRY: A Coarse-grained RNA Model with Sugar Puckering and Noncanonical Base Pairing.

We introduce a new coarse-grained model "CRANBERRY" that incorporates sugar puckering and non-canonical base pairing, two factors central to RNA structure and dynamics, yet rarely included in most coarse-grained models. Our model is parameterized through a contrastive divergence approach, combined with fine-tuning strategies to improve accuracy in generating disordered states, a feature that is critical for the accurate description of thermodynamics. This two-stage training procedure greatly enhances cooperative folding behavior. Due to these advances, CRANBERRY maintains native-like structures with RMSD distributions comparable in scale to those obtained with all-atom force fields. Furthermore, CRANBERRY exhibits better agreement with experimental data on stacking free energies and disordered structures measured by Small Angle X-ray Scattering. In addition, CRANBERRY undergoes repeated folding and unfolding transitions of the ggcGCAAgcc tetraloop, reaching a minimum RMSD of 1.4 Å from an initially extended strand. CRANBERRY predicts melting temperatures in agreement with experimental values, and with a greater cooperativity than all-atom predictions.