bioRxiv : the preprint server for biology · 2024
Defining a highly conserved B cell epitope in the receptor binding motif of SARS-CoV-2 spike glycoprotein.
СтатьяПрепринтНазвание и аннотация: Перевод в очереди
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Malladi Sameer Kumar, Jaiswal Deepika, Ying Baoling, Alsoussi Wafaa B, Darling Tamarand L, Dadonaite Bernadeta, Civljak Alesandro, Horvath Stephen C, Zhou Julian Q, Kim Wooseob, Turner Jackson S, Schmitz Aaron J, Han Fangjie, Scheaffer Suzanne M, Farnsworth Christopher W, Nachbagauer Raffael, Nestorova Biliana, Chalkias Spyros, Klebert Michael K, Edwards Darin K, Paris Robert, Strnad Benjamin S, Middleton William D, O'Halloran Jane A, Presti Rachel M, Bloom Jesse D, Boon Adrianus C M, Diamond Michael S, Bajic Goran, Ellebedy Ali H
Аннотация
SARS-CoV-2 mRNA vaccines induce robust and persistent germinal centre (GC) B cell responses in humans. It remains unclear how the continuous evolution of the virus impacts the breadth of the induced GC B cell response. Using ultrasound-guided fine needle aspiration, we examined draining lymph nodes of nine healthy adults following bivalent booster immunization. We show that 77.8% of the B cell clones in the GC expressed as representative monoclonal antibodies recognized the spike protein, with a third (37.8%) of these targeting the receptor binding domain (RBD). Strikingly, only one RBD-targeting mAb, mAb-52, neutralized all tested SARS-CoV-2 strains, including the recent KP.2 variant. mAb-52 utilizes the IGHV3-66 public clonotype, protects hamsters challenged against the EG.5.1 variant and targets the class I/II RBD epitope, closely mimicking the binding footprint of ACE2. Finally, we show that the remarkable breadth of mAb-52 is due to the somatic hypermutations accumulated within vaccine-induced GC reaction.