American journal of veterinary research · 2026
Calf-related environments are reservoirs of Salmonella enterica on northeastern United States dairy farms with a history of endemic Salmonella Dublin infection.
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Craig Maya J, Cummings Kevin J, Frye Elisha A, Siler Julie D, Franklin-Guild Rebecca J, Sams Kelly L, Cazer Casey L, Goodman Laura B
Аннотация
OBJECTIVE: To identify calf-related environmental reservoirs of Salmonella Dublin on 25 dairy farms with a history of endemic infection in the northeastern US. We hypothesized that maternity pens and calf housing areas were reservoirs of S Dublin on such farms.
METHODS: We used real-time PCR to detect Salmonella enterica in samples collected from dairy farm environments and subsequently cultured PCR-positive samples. All confirmed S Dublin isolates were subjected to antimicrobial susceptibility testing and whole-genome sequencing. A mixed-effects logistic regression model was used to evaluate the association between farm location and PCR positivity.
RESULTS: Of the 678 environmental samples collected, 50.1% were PCR positive for S enterica. A mixed-effects logistic regression model revealed calf-related equipment (OR, 2.7; 95% CI, 1.4 to 3.7) and maternity pen areas (OR, 6.2; 95% CI, 4.0 to 9.8) to have increased odds of testing Salmonella PCR positive in comparison to calf housing areas. Additionally, we isolated and antigenically confirmed S Dublin from maternity pens and calf housing areas. Core genome variation assessment of these isolates revealed high genetic similarity to human isolates.
CONCLUSIONS: The results demonstrate that maternity pens and calf housing areas can harbor S Dublin. Additionally, phylogenetic analysis highlights the potential for this pathogen to spread among human, animal, and environmental reservoirs.
CLINICAL RELEVANCE: Identifying maternity pens and calf housing areas as reservoirs of S Dublin can inform the development of targeted hygiene and biosecurity protocols for dairy herds with endemic infection.
METHODS: We used real-time PCR to detect Salmonella enterica in samples collected from dairy farm environments and subsequently cultured PCR-positive samples. All confirmed S Dublin isolates were subjected to antimicrobial susceptibility testing and whole-genome sequencing. A mixed-effects logistic regression model was used to evaluate the association between farm location and PCR positivity.
RESULTS: Of the 678 environmental samples collected, 50.1% were PCR positive for S enterica. A mixed-effects logistic regression model revealed calf-related equipment (OR, 2.7; 95% CI, 1.4 to 3.7) and maternity pen areas (OR, 6.2; 95% CI, 4.0 to 9.8) to have increased odds of testing Salmonella PCR positive in comparison to calf housing areas. Additionally, we isolated and antigenically confirmed S Dublin from maternity pens and calf housing areas. Core genome variation assessment of these isolates revealed high genetic similarity to human isolates.
CONCLUSIONS: The results demonstrate that maternity pens and calf housing areas can harbor S Dublin. Additionally, phylogenetic analysis highlights the potential for this pathogen to spread among human, animal, and environmental reservoirs.
CLINICAL RELEVANCE: Identifying maternity pens and calf housing areas as reservoirs of S Dublin can inform the development of targeted hygiene and biosecurity protocols for dairy herds with endemic infection.