miRNA profiling identifies biomarkers and regulatory networks involving TGF-β/T-cell pathways in FPIES.
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Gómez-Carballa Alberto, Montoto-Louzao Julián, Viz-Lasheras Sandra, Calle Irene Rivero, Pardo-Seco Jacobo, Bello Xabier, El Zahraa Mallah Nour, Martín Adrián Álvarez, Argiz Laura, Boyle Robert J, Custovic Adnan, Herberg Jethro, Kaforou Myrsini, Arasi Stefania, Fiocchi Alessandro, Pecora Valentina, Barni Simona, Mori Francesca, Bracamonte Teresa, Echeverria Luis, O'Valle-Aísa Virginia, Hernández-Martínez Noelia Lara, Carballeira Iria, García Emilio, Garcia-Magan Carlos, Moure-González José Domingo, Gonzalez-Delgado Purificación, Garriga-Baraut Teresa, Infante Sonsoles, Zambrano-Ibarra Gabriela, Tomás-Pérez Margarita, Machinena Adrianna, Pascal Mariona, Prieto Ana, Vázquez-Cortes Sonia, Fernández-Rivas Montserrat, Vila Leticia, Alsina Laia, Torres María José, Mangone Giusi, González-Matamala Maria-Fernanda, Martinón-Torres Federico, Vázquez-Ortiz Marta, Salas Antonio
Аннотация
OBJECTIVE: To investigate the role of microRNAs (miRNAs) in FPIES and assess their diagnostic potential.
METHODS: We analyzed miRNAs expression profiles in peripheral blood from 29 children with OFC-confirmed moderate to severe FPIES reactions, collected on the OFC day at baseline (TP1), reaction onset (TP2), and four hours post-reaction onset (TP3). Using a hybrid ensemble feature selection approach, we compared these profiles to those of 26 children with acute gastroenteritis (AGE) and sepsis to identify FPIES-specific miRNA signatures.
RESULTS: Longitudinal analysis revealed an early heterogeneous miRNA response that evolved into a more consistent profile in TP3, suggesting a dynamic and evolving regulatory response. Eleven miRNAs emerged as key regulators, highlighting pathways partially shared with other allergic and inflammatory conditions. In silico pathway analysis identified TGF-β signaling and T-cell differentiation as candidate pathways potentially involved in FPIES. Importantly, we discovered and validated 26 miRNAs that accurately distinguish FPIES from sepsis and AGE, including a minimal 2-miRNA diagnostic signature (AUC= 0.98). miRNA-29a correlated with severe FPIES reactions, suggesting involvement in severity mechanisms.
CONCLUSION: Our study reveals novel miRNA-mediated mechanisms in FPIES and identifies candidate biomarkers with diagnostic utility, paving the way for non-invasive molecular tools and therapeutic strategies.