[Anti-exercise fatigue and mechanism of Keap1/Nrf2 pathway in liver of aqueous extract of aronia melanocarpa].

OBJECTIVE

To investigate the anti-fatigue effect of aqueous extract of aronia melanocarpa on exhausted running mice and its mechanism.

METHODS

A mouse model of exhaustive running was used to observe the effects of different doses of aqueous extract of aronia melanocarpa on the time of exhaustive running, and the biochemical indicators such as lactic acid, urea nitrogen and liver/muscle glycogen in the serum of mice were measured to evaluate the anti-exercise-induced fatigue effect in mice. The antioxidant effect of the aqueous extract of aronia melanocarpa was investigated by measuring MDA and SOD. Western blot was used to detect the expression levels of key proteins in the Keap1/Nrf2 antioxidant signaling pathway.

RESULTS

The aqueous extract of aronia melanocarpa could increase the content of liver glycogen and SOD, and reduce the levels of lactic acid, urea nitrogen and MDA by prolonging the exhaustion running time of mice. The expression levels of Keap1 protein in liver tissues induced by exhaustive running were down-regulated, and the expression levels of Nrf2 and HO-1 proteins were up-regulated.

CONCLUSION

The aqueous extract of aronia melanocarpa has anti-fatigue effect, which may be played by activating the Keap1/Nrf2 antioxidant pathway.