Ozone Nanobubble Water as a Sustainable Strategy to Enhance Metabolism, Muscle Function, and Exercise Performance in Mice.

Background/Objectives: Nanobubble water (NBW) is being studied increasingly for its potential benefits in sports nutrition. This study aimed to evaluate whether supplementation with ozone-enriched NBW (O3-NBW) could improve integrated exercise capacity-encompassing endurance performance, muscle strength, and postexercise recovery as well as body composition and metabolic adaptations in mice. Methods: Male ICR mice (n = 24) were allocated into Control, Air-NBW, or O3-NBW (0.2-1 mg/L ozone) groups for 4 weeks. Results: O3-NBW treatment considerably enhanced forelimb grip strength and treadmill running endurance compared to the Control group (both p < 0.05). Analyses of body composition revealed a higher proportion of lean mass and muscle glycogen storage in NBW groups, notably with O3-NBW. Serum markers gathered post-exercise demonstrated a reduction in ammonia and blood urea nitrogen (BUN), suggesting improved nitrogen metabolism. Levels of resting serum creatine kinase (CK) and uric acid were also lower in O3-NBW mice, indicating potential benefits for muscle recovery. In addition, O3-NBW treatment significantly enhanced oxygen consumption (VO2) and reduced the respiratory quotient (RQ), signifying amplified fat oxidation, while also lowering total energy expenditure (all p < 0.05). Spontaneous wheel-running activity remained consistent across all the groups. Conclusions: Taken as a whole, these findings emphasize that O3-NBW supplementation offers ergogenic and metabolic advantages by improving integrated exercise capacity and efficiency of gas exchange, without adverse effects.