iScience · 2021
Standardized two-step testing of antibody activity in COVID-19 convalescent plasma.
СтатьяНазвание и аннотация: Перевод в очереди
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Gilchuk Pavlo, Thomsen Isaac, Yoder Sandra, Brady Eric, Chappell James D, Stevens Laura J, Denison Mark R, Sutton Rachel E, Chen Rita E, VanBlargan Laura A, Suryadevara Naveenchandra, Zost Seth J, Schmitz Jonathan, Pulley Jill M, Diamond Michael S, Rhoads Jillian P, Bernard Gordon R, Self Wesley H, Rice Todd W, Wheeler Allison P, Crowe James E, Carnahan Robert H
Аннотация
The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.