Antithrombotic macrocyclic sesquiterpene pyridine alkaloids from Tripterygium hypoglaucum.

Six previously undescribed macrocyclic sesquiterpene pyridine alkaloids (SPAs) derivatives, named triptocumines A-F (1-6), as well as eighteen known analogs, were isolated from Tripterygium hypoglaucum. The structures were assigned based on analysis of spectroscopic data and electron circular dichroism calculations. Furthermore, compounds 1-6, 8, and 24 could effectively inhibit adenosine diphosphate-induced platelet aggregation, alleviate thrombosis and oxidative stress in zebrafish, reduce endothelin-1 level, protect endothelial cells from oxidative damage, and promote the formation of lumen structure.