Seroprevalence and Risk Factor Assessment of Foot and Mouth Disease Virus in the Pakistan-Afghanistan Border Region.

Foot and mouth disease (FMD) is a highly contagious transboundary viral disease affecting livestock, causing significant economic losses. This sero-epidemiological study investigated FMD distribution and associated risk factors in cattle and buffaloes along the Pakistan-Afghanistan border. A total of 800 serum samples were collected from cattle (n = 610) and buffaloes (n = 190) and tested for antibodies against FMD viral structural proteins (SP) and non-structural proteins (NSP) using ELISA. Overall, 35.25% (282/800) of samples were NSP-positive, indicating natural infection. Serotype-specific analysis showed serotype O as the most prevalent (66.1%), followed by serotype A (50%) and Asia-1 (32%). Cattle exhibited higher FMD prevalence (37%; 95% CI: 33-40) than buffaloes (30%; 95% CI: 23-37). Significant spatial variations in SP and NSP Seroprevalence were observed across different areas. Risk factor analysis identified male sex, young age (1-2 years), crossbred and exotic breeds, summer season, large herd size, smallholders subsistence production systems, poor body condition, and animal movement as factors associated with significantly higher (p < 0.05) FMD circulation. These findings indicate that FMD is highly endemic in the border region and highlight the critical need for government-led mass vaccination campaigns, targeted risk-based surveillance, and stringent movement control to mitigate disease spread. Implementation of such control strategies is essential to safeguard livestock health and protect the regional economy from substantial losses.