Astracondensatols F-L, cycloartane triterpenoids from Astragalus condensatus with their anti-inflammatory potential.

Phytochemical investigation on the roots of Astragalus condensatus (Fabaceae) led to the isolation of seven previously undescribed cycloartane-type triterpenoids, named astracondensatols F-L (1-7), alongside twelve known cycloartane derivatives (8-19) and fifteen other known secondary metabolites (20-34). The structures of these compounds were elucidated through comprehensive spectroscopic analysis, including 1D and 2D NMR, as well as high-resolution electrospray ionization mass spectrometry (HRESIMS). The isolated triterpenoids were evaluated for their effect on nuclear factor kappa B (NF-κB)-driven luciferase expression in THP-1 monocytes stimulated with Escherichia coli lipopolysaccharide (LPS). Five compounds (3, 4, 8, 10, and cyclocephagenol) demonstrated a dose-dependent suppression of NF-κB activation. Notably, these effects were non-selective, as they also inhibited Sp-1 control activity similarly.