Mycotoxin binder for immune and intestinal histopathology ameliorations against Newcastle disease in vaccinated broilers.

BACKGROUND

In broiler farming, vaccination against Newcastle disease (ND) is essential. Nonetheless, during the post-vaccination phase, production may be negatively impacted by mycotoxin contamination in feed. This study aimed to evaluate the effect of mycotoxin binders on immune and intestinal histopathology ameliorations against ND in vaccinated broilers with aflatoxin B1 (AFB1) and ochratoxin A (OTA) toxication.

METHODS

A total of 20 broilers were randomly assigned into 4 groups with 5 replications i.e. (C-) broiler groups with basal feed, (C+) broiler groups with AFB1 and OTA feed contamination, (T1) and (T2) broiler groups with exposed AFB1, OTA, and toxin binders as feed additives with dose 1.1 g/kg and 1.6 g/kg feed, respectively. ND vaccination was carried out on day 7 and 21. Antibody titers were evaluated from serum samples of broiler on days 14, 28, and 35 for further hemagglutination inhibition (HI) test. Histopathology of the cecum and colon organs was evaluated using HE staining on day 36. HI test and histological scoring were analyzed using the One-Way ANOVA, followed by Duncan's test with a p < 0.05 in SPSS v.26 software (IBM Corp., Armonk, NY).

RESULTS

As a result, histopathological improvement of the cecum and colon was reported based on mucosal rupture, hemorrhage and necrosis on day 35. A significant increase in antibody titer was observed on day 35 in the C- and T2 groups, which differed significantly (p < 0.05) from the C+ and T1 groups.

CONCLUSIONS

This study revealed that a 1.6 g/kg toxin binder dose in feed can increase antibody titer and histopathology of cecum and colon in broiler chickens after ND vaccination fed with mycotoxin-contaminated feed.