Automated Antithrombin Activity Detection with Whole Capillary Blood Based on Digital Microfluidic Platform.

UNLABELLED: Antithrombin (AT) plays a crucial role in the human anticoagulant system and has extensive clinical applications. However, traditional detection methods often require large sample volumes, complex procedures, and lengthy processing times.

METHODS

We integrated digital microfluidics technology with AT detection to develop a point-of-care testing (POCT) device that is user-friendly and fully automated for real-time AT testing.

RESULTS

This device allows for automation and enhanced adaptability to various settings, requiring only a minimal sample volume (whole capillary blood), thereby omitting steps such as plasma separation to save time and improve clinical testing efficiency. Comparisons with conventional AT activity detection methods demonstrate a high degree of consistency in the results obtained with this device.

CONCLUSION

The AT detection system we developed exhibits significant effectiveness and holds substantial research potential, positioning it to evolve into a clinically impactful POCT solution for AT assessment.