Magnetic MOF-based sensing platform integrated with graphene field-effect transistors for ultrasensitive detection of infectious disease.

The development of highly sensitive methods for detecting infectious diseases is crucial for preventing disease spread. In this study, a novel sensing platform for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogens was developed by combining a magnetic metal-organic framework (Fe3O4@MIL-100) with graphene field-effect transistors (GFET). The Fe3O4@MIL-100 magnetic MOF was functionalized with SARS-CoV-2-specific antibodies, enabling highly selective pathogen capture in a phosphate-buffered solution. Following magnetic separation, the captured pathogens were detected using GFETs, with a linear detection range of 1 ag/mL to 10 ng/mL and a detection limit as low as 8.60 ag/mL. Furthermore, the platform has been successfully applied to human serum samples, highlighting its remarkable potential for practical application.