Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite.

An electrochemical CCLP-AuNPs (Calcium Cross Linked Pectin with Gold nanoparticles)-based biosensor was developed for sensitive detection and discrimination of carcinoembryonic antigen (CEA) based on dual signal amplification of gold nanoparticles tagged enzymatic catalysis. The electrochemical biosensor was fabricated by assembly of CCLP-AuNp modified GCE and subsequently anti CEA was immobilized on the modified GCE followed by the addition of CEA protein and then forming a sandwich-type conjugate was formed when the biosensor was successively reacted with Au-Anti CEA-HRP(Gold nanoparticles tagged with the antibody Carcinoembryonic antigen labelled with Horse radish peroxidase). In the presence of hydroquinone and hydrogen peroxide, in the PB(phosphate buffer) solution resulted in the fabrication of biosensor. The concentration of CEA in the range of 0.1 ng/mL to 10 ng/mL with low detection limit of 0.08 ng/mL thus the fabricated biosensor proves to be feasible tool for clinical diagnosis in complex biological systems and shows good for the early diagnosis of cancer.