Enhanced Immune Functions of In Vitro Human Natural Killer Cells and Splenocytes in Immunosuppressed Mice Supplemented with Mature Silkworm Products.

OBJECTIVES

The immune-enhancing properties of steamed mature silkworm, known as HongJam (HJ), were investigated using human interleukin-2-independent Natural Killer 92 (NK92-MI) cells and a cyclophosphamide intraperitoneal injection-induced immunosuppressed mice model (CPA-IP). White Jade variety mature silkworm HJ (WJ-HJ) was used to prepare WJ-HJ supercritical fluid extracts (WJ-SCE) and WJ-HJ-supplemented feeds.

RESULTS

Treatment with WJ-SCE significantly enhanced proliferation, migration, and cytotoxicity of NK92-MI cells against various cancer cells while improving mitochondrial function and ATP production (p < 0.05). In CPA-IP mice, consumption of WJ-HJ-supplemented feeds restored immune function by improving body weight, immune organ indices, immunoglobulin levels, and blood cytokines. Splenocyte proliferation and cytotoxicity were significantly elevated in both saline intraperitoneal injection (Sal-IP) and CPA-IP groups with WJ-HJ supplementation, independent of mitogen activation (p < 0.05).

CONCLUSIONS

These results suggest that WJ-HJ enhances immune modulation and immune surveillance functions of NK cells by improving mitochondrial and cytotoxic functions. WJ-HJ holds promise as a functional food for immune enhancement, pending clinical validation.