Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2024
Simple enzyme based fluorimetric biosensor for urea in human biofluids.
СтатьяНазвание и аннотация: Перевод в очереди
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Servarayan Karthika Lakshmi, Sundaram Ellairaja, Velayutham Kumaravel, Aravind Manikka Kubendran, Sundarapandi Manickam, Ashokkumar Balasubramaniem, Sivasamy Vasantha Vairathevar
Аннотация
As an important biomarker for renal related diseases, detection of urea is playing a vital role in human biofluids on clinical diagnosis concern. In this work, a synthetic salicyaldehyde based imine fluorophore was synthesized using sonication method and conjugated with urease which was used as fluorescent biosensor for the detection of urea in serum samples. This enzyme based biosensor has shown a good selectivity and sensitivity towards urea with the linear range from 2 to 80 mM and the detection limit of 73 µM. The sensing response obtain is highly agreeing with existing analytical technique for urea detection which strongly recommends this biosensor for clinical application.