The Journal of trauma · 2005
Effect of albumin and Hespan on rodent hepatocyte function after hemorrhagic shock and sepsis.
Оценочное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Vlahos Angie L, Crawford Robert S, Matthew Howard T, Ledgerwood Anna M, Lucas Charles E
Аннотация
BACKGROUND: Sepsis and hemorrhagic shock (HS) cause hypoalbuminemia, with extravascular albumin relocation. Prior studies on cultured rat hepatocytes showed a fall in albumin genesis when exposed to albumin and Hespan (HES) supplementation. This study assesses the effects of increased colloid osmotic pressure on albumin and urea genesis from hepatocytes exposed to HS or septic insult before harvesting.
METHODS: Hepatocyte synthesis of albumin was measured in an in vitro collagen sandwich preparation with a seeding density of 1 million cells per dish in Dulbecco's modified Eagle's medium without additional colloid (control cultures). Study groups included control plus 2% bovine serum albumin (BSA), 4% BSA, 2% HES, and 4% HES.
RESULTS: The albumin secretion in the control cultures of HS cells rose from 19.03 microg/d/10(6) cells on day 3 to 88.81 microg/d/10(6) cells by day 8 and remained constant. In contrast, albumin genesis in the 2% and 4% BSA groups rose from a significantly higher (p < 0.05) day 3 value of 39.83 microg/d/10(6) cells and 48.76 microg/d/10(6) cells, respectively, to 116.39 microg/d/10(6) cells and 118.76 microg/d/10(6) cells by day 8 and remained fairly constant. Albumin genesis from the HS cells after 2% HES and 4% HES was similar to control HS cells through day 8 but was significantly greater by day 12. Urea genesis from HS cells was greater in the 4% HES group compared with control and the 2% BSA and 4% BSA groups. The albumin genesis in the control cultures of septic cells was 9.32 microg/d/10(6) cells on day 3, rose to 81.46 microg/d/10(6) cells by day 8, and remained fairly constant. The BSA-supplemented septic cells had significantly higher albumin output than control cells early in the experiment, whereas the 2% HES group had higher albumin secretion than control cells throughout the study.
CONCLUSION: HS and septic cultured hepatocytes respond to a rise in interstitial colloid osmotic pressure with a rise in albumin genesis, associated with morphologic changes typical of cellular injury. The mechanism remains cryptic; studies on human hepatocytes are needed.
METHODS: Hepatocyte synthesis of albumin was measured in an in vitro collagen sandwich preparation with a seeding density of 1 million cells per dish in Dulbecco's modified Eagle's medium without additional colloid (control cultures). Study groups included control plus 2% bovine serum albumin (BSA), 4% BSA, 2% HES, and 4% HES.
RESULTS: The albumin secretion in the control cultures of HS cells rose from 19.03 microg/d/10(6) cells on day 3 to 88.81 microg/d/10(6) cells by day 8 and remained constant. In contrast, albumin genesis in the 2% and 4% BSA groups rose from a significantly higher (p < 0.05) day 3 value of 39.83 microg/d/10(6) cells and 48.76 microg/d/10(6) cells, respectively, to 116.39 microg/d/10(6) cells and 118.76 microg/d/10(6) cells by day 8 and remained fairly constant. Albumin genesis from the HS cells after 2% HES and 4% HES was similar to control HS cells through day 8 but was significantly greater by day 12. Urea genesis from HS cells was greater in the 4% HES group compared with control and the 2% BSA and 4% BSA groups. The albumin genesis in the control cultures of septic cells was 9.32 microg/d/10(6) cells on day 3, rose to 81.46 microg/d/10(6) cells by day 8, and remained fairly constant. The BSA-supplemented septic cells had significantly higher albumin output than control cells early in the experiment, whereas the 2% HES group had higher albumin secretion than control cells throughout the study.
CONCLUSION: HS and septic cultured hepatocytes respond to a rise in interstitial colloid osmotic pressure with a rise in albumin genesis, associated with morphologic changes typical of cellular injury. The mechanism remains cryptic; studies on human hepatocytes are needed.