Screening of symbiotic Streptomyces spp. and optimization of microalgal growth in a microalgae-actinomycetes co-culture system.

Microalgal biodiesel as a substitute for fossil energy has attracted extensive attention. However, the high cost of microalgae cultivation limits the industrial production of microalgal biodiesel. The co-culture system may offer a means to increase microalgae's biomass production. In this study, Streptomyces strains were selected to construct and optimize co-culture systems with Monoraphidium sp. HDMA-11 and the algal cell biomass, lipid content, phycocyanin content, starch content, and fatty acid composition were determined. The results showed that Streptomyces nojiriensis significantly promoted Monoraphidium sp. HDMA-11 growth and a co-culture system were established. Orthogonal experiments showed that the Monoraphidium sp. HDMA-11 biomass was further increased when the initial culture pH was 7.5, the inoculation time of Streptomyces strain supernatants was 36 h, the volume ratio of microalgal actinomycetes was 1:1, and no additional acetic acid was added. Under these conditions, compared with monocultured Monoraphidium sp. HDMA-11, the cell biomass and lipid productivity of the co-culture system increased by 525.8 and 155.1%, respectively. These results suggest that S. nojiriensis supernatant potentially enhances microalgae biomass and may represent a new method to improve microalgae growth.