Flow-injection chemiluminescence determination of olanzapine using N-chlorosuccinimide-calcein reaction sensitized by zinc (II).

A novel, rapid and sensitive chemiluminescence (CL) method combined with flow-injection (FI) has been established for the estimation of olanzapine. This method is based on the CL signal generated between N-chlorosuccinimide and olanzapine in an alkaline medium in the presence of calcein and Zn(II). Under optimum conditions, the CL signal was proportional to the olanzapine concentration ranging from 1.0 × 10(-10) to 3.0 × 10(-7) g/mL. The detection limit is 8.9 × 10(-11) g/mL olanzapine (3σ) and the relative standard deviation for 3.0 × 10(-9) g/mL of olanzapine is 1.9% (n = 11). The current CL method was applied to determine olanzapine in pharmaceutical formulations and biological fluids with satisfactory results. The possible CL reaction mechanism is discussed briefly.