Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2008
[Homing of CFSE-labeled antigen-specific CD4(+)CD25(+) regulatory T cells in islets transplantation].
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Zhang Mei, Xu Shu-Hang, Xu Yu, Liu Cui-Ping, Mao Xiao-Dong, Xu Kuan-Feng, Liu Chao
Аннотация
AIM: To observe the distribution and proliferation of the antigen-specific CD4(+)CD25(+) regulatory T (Treg ) cells and investigate the potential mechanism of antigen-specific CD4(+)CD25(+) regulatory T cells on the suppression of rejection for allogenetic islet transplantation in vivo.
METHODS: Antigen-specific CD4(+)CD25(+) Treg cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. After labeled by CFSE, 8x10(5) antigen-specific Treg cells were injected via tail vein with islets transplantation. Homing and distribution of antigen-specific CD4(+)CD25(+) Treg cells were investigated by flow cytometric analysis and immunofluorescence.
RESULTS: In vivo, the mean survival time of recipients with islets and antigen-specific CD4(+)CD25(+) Treg cells were (34.57+/-17.15) days, whereas transplanted islets without Treg treatment survived (10.6+/-1.82) days in control mice. The transferred antigen-specific CD4(+)CD25(+) Treg cells were mainly resident in pancreatic node and mesenteric node.
CONCLUSION: Allogeneic antigen-specific CD4(+)CD25(+) Treg cells prolong islet graft survival, and draining lymphoid tissue play a key role in the immune response.
METHODS: Antigen-specific CD4(+)CD25(+) Treg cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. After labeled by CFSE, 8x10(5) antigen-specific Treg cells were injected via tail vein with islets transplantation. Homing and distribution of antigen-specific CD4(+)CD25(+) Treg cells were investigated by flow cytometric analysis and immunofluorescence.
RESULTS: In vivo, the mean survival time of recipients with islets and antigen-specific CD4(+)CD25(+) Treg cells were (34.57+/-17.15) days, whereas transplanted islets without Treg treatment survived (10.6+/-1.82) days in control mice. The transferred antigen-specific CD4(+)CD25(+) Treg cells were mainly resident in pancreatic node and mesenteric node.
CONCLUSION: Allogeneic antigen-specific CD4(+)CD25(+) Treg cells prolong islet graft survival, and draining lymphoid tissue play a key role in the immune response.