Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2007
[Effects on T cell proliferation by culture supernatant of fibroblast cells modified with mIDO gene].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Luo Lu, Zeng Yao-Ying, Wang Zhen, Wang Qing, Zhao Li, Yang Dong-Zi
Аннотация
AIM: To investigate the effects on T cell proliferation by culture supernatant of fibroblast cells modified by mIDO gene.
METHODS: Eukaryotic expression plasmid pEGFP-Nl-mlDO was transfected into the mouse fibroblast cell lines by liposome. The concentration of tryptophan in pEGFP-Nl-mIDO fibroblast cell culture medium was tested. The effects on ConA-stimulated T cells, proliferation by pEGFP-Nl-mlDO fibroblast cell culture medium were analyzed by CFSE technique.
RESULTS: pEGFP-N1-mlDO was transfected into the fibroblasts cell lines successfully. Tryptophan in pEGFP-Nl-mIDO-transfected-fibroblast cell culture medium was undetectable, which showed pEGFP-Nl-mIDO-transfected-fibroblast cells produced active mIDO, resulting in the degradation of tryptophan in the medium. mIDO could inhibit proliferation of ConA-stimulated T cells, which was marked by CFSE.
CONCLUSION: T cell proliferation can be inhibited by the fibroblast cells modified by mIDO gene.
METHODS: Eukaryotic expression plasmid pEGFP-Nl-mlDO was transfected into the mouse fibroblast cell lines by liposome. The concentration of tryptophan in pEGFP-Nl-mIDO fibroblast cell culture medium was tested. The effects on ConA-stimulated T cells, proliferation by pEGFP-Nl-mlDO fibroblast cell culture medium were analyzed by CFSE technique.
RESULTS: pEGFP-N1-mlDO was transfected into the fibroblasts cell lines successfully. Tryptophan in pEGFP-Nl-mIDO-transfected-fibroblast cell culture medium was undetectable, which showed pEGFP-Nl-mIDO-transfected-fibroblast cells produced active mIDO, resulting in the degradation of tryptophan in the medium. mIDO could inhibit proliferation of ConA-stimulated T cells, which was marked by CFSE.
CONCLUSION: T cell proliferation can be inhibited by the fibroblast cells modified by mIDO gene.