The American journal of tropical medicine and hygiene · 2021
First Record of Natural Transovarial Transmission of Dengue Virus in Aedes albopictus from Cuba.
СтатьяНазвание и аннотация: Перевод в очереди
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Piedra Luis Augusto, Martinez Liss Claudia, Ruiz Armando, Vazquez Juan Ramon, Guzman Maria Guadalupe, Rey Jorge, Bisset Juan Andres
Аннотация
Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.