FEBS letters · 1991
The preparation of biotinyl-epsilon-aminocaproylated forms of the vasoactive intestinal polypeptide (VIP) as probes for the VIP receptor.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Andersson M, Marie J C, Carlquist M, Mutt V
Аннотация
Vasoactive intestinal polypeptide (VIP) was biotinyl-epsilon-aminocaproylated using sulfosuccinimidyl-6-(biotinamido) hexanoate thereby producing a series of products that were separated by high performance liquid chromatography (HPLC). Seven VIP-derivatives were isolated and the number and location of biotinyl-epsilon-aminocaproylation was determined by a combination of enzymatic degradation and plasma desorption mass spectrometry (PDMS). Receptor binding experiments with the VIP biotinyl-epsilon-aminocaproylated derivatives revealed IC50 values for the monobiotinyl-epsilon-aminocaproylated peptides that were 1.3-3.2 times higher than for natural VIP. All isolated biotinyl-epsilon-aminocaproylated derivatives possess VIP-like bioactivity as shown by an assay measuring pancreatic juice secretion in cat, VIP biotinyl-epsilon-aminocaproylated in position lysine being almost equipotent with natural VIP.
Предметные рубрики MeSH
Amino Acid SequenceAnimalsBinding, CompetitiveBiotinCell MembraneChromatography, High Pressure LiquidCross-Linking ReagentsLiverMass SpectrometryMolecular ProbesMolecular Sequence DataReceptors, Gastrointestinal HormoneReceptors, Vasoactive Intestinal PeptideSuccinimidesSwineVasoactive Intestinal Peptide