[Effect of insulin on retinal glycogen content].

BACKGROUND

The effect of insulin on glucose and glycogen metabolism in peripheral organs is well known. However, information about the action of this peptide in the retina is incomplete. We addressed the questions whether insulin influences glycogen content in the cat retina and whether glycogen breakdown is triggered by lack of glucose.

MATERIAL AND

METHODS

Eyes from adult cats were enucleated under deep barbiturate and fentanylanesthesia. Retinas were snap frozen either before or following arterial in vitro perfusion. Three conditions were studied: a) Perfusion with a glucose- and insulin-free medium; b) perfusion with the addition of physiologic glucose concentration; and c) in combination with insulin. Glycogen content was determined by in vitro measurement of glucose converted from glycogen.

RESULTS

The reference value for retinal glycogen after enucleation (10 min of ischemia) is 2.4 micrograms glucose/mg protein. Glucose- and insulin-free perfusion for 80 min following "normoglycemia" reduced the amount of retinal glycogen by one third. Perfusion for 3 h with 5.5 mM glucose led to a small increase of the partly depleted glycogen stores. Insulin, in contrast, markedly augmented the glycogen content.

CONCLUSIONS

Insulin led to an increase in retinal glycogen content, indicating an influence of this peptide on retinal glucose and glycogen metabolism. However, it appears that glycogen might play a dynamic role in retinal metabolism as a buffer between abrupt changes in focal metabolic demands that occur during normal glucose supply rather than acting solely as an emergency energy reserve for neural function during hypoglycemia.